Abstract
Numerous industries discharge substantial amounts of carbon monoxide (CO) into the atmosphere as waste; utilizing CO-containing industrial waste gases to produce useful organic chemicals has recently attracted attention. Here, we constructed a two-stage biocatalytic CO-conversion system for producing poly-3-hydroxybutyrate (PHB), a promising degradable biopolymer. In the first stage, Acetobacterium woodii, an acetogenic bacterial strain containing CO dehydrogenase (CODH) and formate dehydrogenase (FDH), was used as a whole-cell biocatalyst to transform CO into formate independent of an external reducing agent, such as H2. The conversion yield and specificity were close to 100% when the strain's energy metabolism was blocked to suppress acetate production. The resulting formate was fed to a second bioreactor, where it was converted to PHB by engineered Methylbacterium extorquens AM1. The two-stage bioconversion of CO to a valuable product via formate as an intermediate offers a novel and promising strategy for CO utilization.
Original language | English |
---|---|
Article number | 124394 |
Journal | Chemical Engineering Journal |
Volume | 389 |
DOIs | |
Publication status | Published - 2020 Jun 1 |
Bibliographical note
Funding Information:This research was supported by the C1 Gas Refinery Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning ( 2015M3D3A1A01064919 ) and KCRC2020 (2014M1A8A10492).
Publisher Copyright:
© 2020 Elsevier B.V.
Keywords
- Biopolymer
- Carbon monoxide
- Formate
- Poly-3-hydroxybutyrate
- Two-stage bioconversion
ASJC Scopus subject areas
- General Chemistry
- Environmental Chemistry
- General Chemical Engineering
- Industrial and Manufacturing Engineering