Two-stage bioconversion of carbon monoxide to biopolymers via formate as an intermediate

Ho Won Hwang; Jihee Yoon; Kyoungseon Min; Min Sik Kim; Seung Jin Kim; Dae Haeng Cho; Hadiyati Susila; Jeong Geol Na; Min Kyu Oh; Yong Hwan Kim

doi:10.1016/j.cej.2020.124394

Two-stage bioconversion of carbon monoxide to biopolymers via formate as an intermediate

Ho Won Hwang, Jihee Yoon, Kyoungseon Min, Min Sik Kim, Seung Jin Kim, Dae Haeng Cho, Hadiyati Susila, Jeong Geol Na, Min Kyu Oh, Yong Hwan Kim

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

Numerous industries discharge substantial amounts of carbon monoxide (CO) into the atmosphere as waste; utilizing CO-containing industrial waste gases to produce useful organic chemicals has recently attracted attention. Here, we constructed a two-stage biocatalytic CO-conversion system for producing poly-3-hydroxybutyrate (PHB), a promising degradable biopolymer. In the first stage, Acetobacterium woodii, an acetogenic bacterial strain containing CO dehydrogenase (CODH) and formate dehydrogenase (FDH), was used as a whole-cell biocatalyst to transform CO into formate independent of an external reducing agent, such as H₂. The conversion yield and specificity were close to 100% when the strain's energy metabolism was blocked to suppress acetate production. The resulting formate was fed to a second bioreactor, where it was converted to PHB by engineered Methylbacterium extorquens AM1. The two-stage bioconversion of CO to a valuable product via formate as an intermediate offers a novel and promising strategy for CO utilization.

Original language	English
Article number	124394
Journal	Chemical Engineering Journal
Volume	389
DOIs	https://doi.org/10.1016/j.cej.2020.124394
Publication status	Published - 2020 Jun 1

Bibliographical note

Funding Information:
This research was supported by the C1 Gas Refinery Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning ( 2015M3D3A1A01064919 ) and KCRC2020 (2014M1A8A10492).

Publisher Copyright:
© 2020 Elsevier B.V.

Keywords

Biopolymer
Carbon monoxide
Formate
Poly-3-hydroxybutyrate
Two-stage bioconversion

ASJC Scopus subject areas

General Chemistry
Environmental Chemistry
General Chemical Engineering
Industrial and Manufacturing Engineering

Access to Document

10.1016/j.cej.2020.124394

Cite this

@article{6de9e0934e3b406081000498ad3cce7d,

title = "Two-stage bioconversion of carbon monoxide to biopolymers via formate as an intermediate",

abstract = "Numerous industries discharge substantial amounts of carbon monoxide (CO) into the atmosphere as waste; utilizing CO-containing industrial waste gases to produce useful organic chemicals has recently attracted attention. Here, we constructed a two-stage biocatalytic CO-conversion system for producing poly-3-hydroxybutyrate (PHB), a promising degradable biopolymer. In the first stage, Acetobacterium woodii, an acetogenic bacterial strain containing CO dehydrogenase (CODH) and formate dehydrogenase (FDH), was used as a whole-cell biocatalyst to transform CO into formate independent of an external reducing agent, such as H2. The conversion yield and specificity were close to 100% when the strain's energy metabolism was blocked to suppress acetate production. The resulting formate was fed to a second bioreactor, where it was converted to PHB by engineered Methylbacterium extorquens AM1. The two-stage bioconversion of CO to a valuable product via formate as an intermediate offers a novel and promising strategy for CO utilization.",

keywords = "Biopolymer, Carbon monoxide, Formate, Poly-3-hydroxybutyrate, Two-stage bioconversion",

author = "Hwang, {Ho Won} and Jihee Yoon and Kyoungseon Min and Kim, {Min Sik} and Kim, {Seung Jin} and Cho, {Dae Haeng} and Hadiyati Susila and Na, {Jeong Geol} and Oh, {Min Kyu} and Kim, {Yong Hwan}",

note = "Funding Information: This research was supported by the C1 Gas Refinery Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning ( 2015M3D3A1A01064919 ) and KCRC2020 (2014M1A8A10492). Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2020",

month = jun,

day = "1",

doi = "10.1016/j.cej.2020.124394",

language = "English",

volume = "389",

journal = "Chemical Engineering Journal",

issn = "1385-8947",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Two-stage bioconversion of carbon monoxide to biopolymers via formate as an intermediate

AU - Hwang, Ho Won

AU - Yoon, Jihee

AU - Min, Kyoungseon

AU - Kim, Min Sik

AU - Kim, Seung Jin

AU - Cho, Dae Haeng

AU - Susila, Hadiyati

AU - Na, Jeong Geol

AU - Oh, Min Kyu

AU - Kim, Yong Hwan

N1 - Funding Information: This research was supported by the C1 Gas Refinery Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT & Future Planning ( 2015M3D3A1A01064919 ) and KCRC2020 (2014M1A8A10492). Publisher Copyright: © 2020 Elsevier B.V.

PY - 2020/6/1

Y1 - 2020/6/1

N2 - Numerous industries discharge substantial amounts of carbon monoxide (CO) into the atmosphere as waste; utilizing CO-containing industrial waste gases to produce useful organic chemicals has recently attracted attention. Here, we constructed a two-stage biocatalytic CO-conversion system for producing poly-3-hydroxybutyrate (PHB), a promising degradable biopolymer. In the first stage, Acetobacterium woodii, an acetogenic bacterial strain containing CO dehydrogenase (CODH) and formate dehydrogenase (FDH), was used as a whole-cell biocatalyst to transform CO into formate independent of an external reducing agent, such as H2. The conversion yield and specificity were close to 100% when the strain's energy metabolism was blocked to suppress acetate production. The resulting formate was fed to a second bioreactor, where it was converted to PHB by engineered Methylbacterium extorquens AM1. The two-stage bioconversion of CO to a valuable product via formate as an intermediate offers a novel and promising strategy for CO utilization.

AB - Numerous industries discharge substantial amounts of carbon monoxide (CO) into the atmosphere as waste; utilizing CO-containing industrial waste gases to produce useful organic chemicals has recently attracted attention. Here, we constructed a two-stage biocatalytic CO-conversion system for producing poly-3-hydroxybutyrate (PHB), a promising degradable biopolymer. In the first stage, Acetobacterium woodii, an acetogenic bacterial strain containing CO dehydrogenase (CODH) and formate dehydrogenase (FDH), was used as a whole-cell biocatalyst to transform CO into formate independent of an external reducing agent, such as H2. The conversion yield and specificity were close to 100% when the strain's energy metabolism was blocked to suppress acetate production. The resulting formate was fed to a second bioreactor, where it was converted to PHB by engineered Methylbacterium extorquens AM1. The two-stage bioconversion of CO to a valuable product via formate as an intermediate offers a novel and promising strategy for CO utilization.

KW - Biopolymer

KW - Carbon monoxide

KW - Formate

KW - Poly-3-hydroxybutyrate

KW - Two-stage bioconversion

UR - http://www.scopus.com/inward/record.url?scp=85079409444&partnerID=8YFLogxK

U2 - 10.1016/j.cej.2020.124394

DO - 10.1016/j.cej.2020.124394

M3 - Article

AN - SCOPUS:85079409444

SN - 1385-8947

VL - 389

JO - Chemical Engineering Journal

JF - Chemical Engineering Journal

M1 - 124394

ER -

Two-stage bioconversion of carbon monoxide to biopolymers via formate as an intermediate

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this