TY - JOUR
T1 - Tyrosinase-immobilized CNT based biosensor for highly-sensitive detection of phenolic compounds
AU - Wee, Youngho
AU - Park, Seunghwan
AU - Kwon, Young Hyeon
AU - Ju, Youngjun
AU - Yeon, Kyung Min
AU - Kim, Jungbae
N1 - Funding Information:
This work was supported by the Global Research Laboratory Program ( 2014K1A1A2043032 ) and Nano-Material Technology Development Program ( 2014M3A7B4052193 ) through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT and Future Planning . This work was also supported by the Korea Institute of Energy Technology Evaluation and Planning ( KETEP ) and the Ministry of Trade, Industry & Energy ( MOTIE ) of the Republic of Korea ( 20182010600430 ).
PY - 2019/5/1
Y1 - 2019/5/1
N2 - Highly sensitive phenol biosensor was developed by using well-dispersed carbon nanotubes (CNTs) in enzyme solution and adding CNTs in enzyme electrodes. First, the intact CNTs were dispersed in aqueous tyrosinase (TYR) solution, and TYR molecules were precipitated and crosslinked to prepare the sample of enzyme adsorption, precipitation and crosslinking (EAPC). EAPC exhibited 10.5- and 5.4-fold higher TYR activity per mg of CNTs as compared to enzyme adsorption (EA) and enzyme adsorption/crosslinking (EAC), respectively. EAPC retained 29% of its initial activity after incubation at 40 °C for 128 h, while EA and EAC showed no residual activities, respectively. In biosensing a model phenolic compound of catechol, the sensitivities of EA, EAC and EAPC electrodes on glassy carbon electrode (GCE) were 34, 281 and 675 µA/mM/cm 2 , respectively. When 90 w/w% CNTs were added to the enzyme electrodes, the sensitivities of EA, EAC, and EAPC electrodes were 146, 427, and 1160 µA/mM/cm 2 , respectively, and the EAPC electrode showed a 2.3-fold increase in sensitivity upon CNT addition. Catechol and phenol could also be detected by EAPC on the screen-printed electrode (SPE), with sensitivities of 1340 and 1170 µA/mM/cm 2 , respectively. The sensitivity of EAPC-SPE for phenol detection in the effluent from real municipal wastewater treatment plant was 1100 µA/mM/cm 2 . The sensitivity of EAPC-SPE retained 74% of its initial sensitivity after incubation at 40 °C for 12 h. The combination of EAPC immobilization and CNT addition has great potential for application in the development of sensitive enzyme biosensors for various analytes and phenols in water environments.
AB - Highly sensitive phenol biosensor was developed by using well-dispersed carbon nanotubes (CNTs) in enzyme solution and adding CNTs in enzyme electrodes. First, the intact CNTs were dispersed in aqueous tyrosinase (TYR) solution, and TYR molecules were precipitated and crosslinked to prepare the sample of enzyme adsorption, precipitation and crosslinking (EAPC). EAPC exhibited 10.5- and 5.4-fold higher TYR activity per mg of CNTs as compared to enzyme adsorption (EA) and enzyme adsorption/crosslinking (EAC), respectively. EAPC retained 29% of its initial activity after incubation at 40 °C for 128 h, while EA and EAC showed no residual activities, respectively. In biosensing a model phenolic compound of catechol, the sensitivities of EA, EAC and EAPC electrodes on glassy carbon electrode (GCE) were 34, 281 and 675 µA/mM/cm 2 , respectively. When 90 w/w% CNTs were added to the enzyme electrodes, the sensitivities of EA, EAC, and EAPC electrodes were 146, 427, and 1160 µA/mM/cm 2 , respectively, and the EAPC electrode showed a 2.3-fold increase in sensitivity upon CNT addition. Catechol and phenol could also be detected by EAPC on the screen-printed electrode (SPE), with sensitivities of 1340 and 1170 µA/mM/cm 2 , respectively. The sensitivity of EAPC-SPE for phenol detection in the effluent from real municipal wastewater treatment plant was 1100 µA/mM/cm 2 . The sensitivity of EAPC-SPE retained 74% of its initial sensitivity after incubation at 40 °C for 12 h. The combination of EAPC immobilization and CNT addition has great potential for application in the development of sensitive enzyme biosensors for various analytes and phenols in water environments.
KW - Carbon nanotubes
KW - Enzymatic biosensor
KW - Enzyme adsorption, precipitation and crosslinking
KW - Phenolic compounds, Screen-printed electrode
KW - Tyrosinase
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U2 - 10.1016/j.bios.2019.03.008
DO - 10.1016/j.bios.2019.03.008
M3 - Article
C2 - 30884314
AN - SCOPUS:85062809951
SN - 0956-5663
VL - 132
SP - 279
EP - 285
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -