Tyrosine-mediated two-dimensional peptide assembly and its role as a bio-inspired catalytic scaffold

Hyung Seok Jang, Jung Ho Lee, Yong Sun Park, Young O. Kim, Jimin Park, Tae Youl Yang, Kyoungsuk Jin, Jaehun Lee, Sunghak Park, Jae Myoung You, Ki Woong Jeong, Areum Shin, In Seon Oh, Min Kyung Kwon, Yong Il Kim, Hoon Hwe Cho, Heung Nam Han, Yangmee Kim, Yoon Ho Chang, Seung R. PaikKi Tae Nam, Yoon Sik Lee

Research output: Contribution to journalArticlepeer-review

108 Citations (Scopus)

Abstract

In two-dimensional interfacial assemblies, there is an interplay between molecular ordering and interface geometry, which determines the final morphology and order of entire systems. Here we present the interfacial phenomenon of spontaneous facet formation in a water droplet driven by designed peptide assembly. The identified peptides can flatten the rounded top of a hemispherical droplet into a plane by forming a macroscopic two-dimensional crystal structure. Such ordering is driven by the folding geometry of the peptide, interactions of tyrosine and crosslinked stabilization by cysteine. We discover the key sequence motifs and folding structures and study their sequence-specific assembly. The well-ordered, densely packed, redox-active tyrosine units in the YYACAYY (H-Tyr-Tyr-Ala-Cys-Ala-Tyr-Tyr-OH) film can trigger or enhance chemical/electrochemical reactions, and can potentially serve as a platform to fabricate a molecularly tunable, self-repairable, flat peptide or hybrid film.

Original languageEnglish
Article number3665
JournalNature communications
Volume5
DOIs
Publication statusPublished - 2014 Apr 11
Externally publishedYes

Bibliographical note

Funding Information:
We thank Hye-Rim So and Professor Han-Bin Oh in Sogang University for MALDI analysis. We also appreciate the comments from Professor Romas Kazlauskas in the University of Minnesota. This work is supported by the National Research Foundation of Korea (NRF) under the Ministry of Science, ICT and Future. We gratefully acknowledge the support of the Pioneer Research Center Program (2011-0027888) of NRF. K.T.N. thanks the support of the Basic Science Research (2011-0011225) and Global Frontier R&D program on Center for Multiscale Energy System (0420-20130104) and the Fusion Research Program for Green Technologies through NRF the National Research Foundation of Korea(NRF) funded by the Ministry of Science, ICT & Future (2012M3C1A1048863). H.N.H. was supported by Basic Science Research Programme through the National Research Foundation in Korea funded by the Ministry of Science, ICT and Future Planning (2013008806).

ASJC Scopus subject areas

  • General Chemistry
  • General Biochemistry,Genetics and Molecular Biology
  • General Physics and Astronomy

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