Ultra-High Performance Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for the Determination of Caffeine in Energy Drinks

Min Soo Lee; Nguyen Lan Huong; Nguyen Huu Hoang; Anil Shrestha; Je Won Park

doi:10.1080/00032719.2014.888730

Ultra-High Performance Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for the Determination of Caffeine in Energy Drinks

Min Soo Lee
, Nguyen Lan Huong
, Nguyen Huu Hoang
, Anil Shrestha
, Je Won Park^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A simple and highly sensitive method for the determination of caffeine in energy beverages was developed and validated. Sample preparation utilizing solid-phase extraction (SPE) was simple and reliable. Separation by isocratic ultra-high performance liquid chromatography (UPLC) with a reversed-phase C₁₈ column was performed within 6 min. The use of SPE with UPLC coupled with electrospray ionization-multiple tandem mass spectrometry detection (ESI-MSⁿ) was accurate, reproducible, and validated for the determination of caffeine in energy drink matrices. The limit of quantification for caffeine was approximately 2.1 ng mL^-1. The intra-day and inter-day precisions were less than 4%, and the accuracy of the measurements was between 85.1% and 93.2%. Results for caffeine concentrations in eighteen beverages were compared to the values on the labels. This paper describes the first use of the UPLC-ESI-ion trap MSⁿ technique for quality-control purposes of caffeine present in energy drinks.

Original language	English
Pages (from-to)	1852-1861
Number of pages	10
Journal	Analytical Letters
Volume	47
Issue number	11
DOIs	https://doi.org/10.1080/00032719.2014.888730
Publication status	Published - 2014 Jul
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Ministry of Education, Science, and Technology (MEST) (20122039424), and the grant (PJ0094834) funded by the Next-Generation BioGreen21 program, Rural Development Administration.

Keywords

Caffeine
Energy drinks
HPLC-ESI-MS/MS
Solid-phase extraction

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

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10.1080/00032719.2014.888730

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title = "Ultra-High Performance Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for the Determination of Caffeine in Energy Drinks",

abstract = "A simple and highly sensitive method for the determination of caffeine in energy beverages was developed and validated. Sample preparation utilizing solid-phase extraction (SPE) was simple and reliable. Separation by isocratic ultra-high performance liquid chromatography (UPLC) with a reversed-phase C18 column was performed within 6 min. The use of SPE with UPLC coupled with electrospray ionization-multiple tandem mass spectrometry detection (ESI-MSn) was accurate, reproducible, and validated for the determination of caffeine in energy drink matrices. The limit of quantification for caffeine was approximately 2.1 ng mL-1. The intra-day and inter-day precisions were less than 4\%, and the accuracy of the measurements was between 85.1\% and 93.2\%. Results for caffeine concentrations in eighteen beverages were compared to the values on the labels. This paper describes the first use of the UPLC-ESI-ion trap MSn technique for quality-control purposes of caffeine present in energy drinks.",

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author = "Lee, \{Min Soo\} and Huong, \{Nguyen Lan\} and Hoang, \{Nguyen Huu\} and Anil Shrestha and \{Won Park\}, Je",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Ministry of Education, Science, and Technology (MEST) (20122039424), and the grant (PJ0094834) funded by the Next-Generation BioGreen21 program, Rural Development Administration.",

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doi = "10.1080/00032719.2014.888730",

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T1 - Ultra-High Performance Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for the Determination of Caffeine in Energy Drinks

AU - Lee, Min Soo

AU - Huong, Nguyen Lan

AU - Hoang, Nguyen Huu

AU - Shrestha, Anil

AU - Won Park, Je

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Ministry of Education, Science, and Technology (MEST) (20122039424), and the grant (PJ0094834) funded by the Next-Generation BioGreen21 program, Rural Development Administration.

PY - 2014/7

Y1 - 2014/7

N2 - A simple and highly sensitive method for the determination of caffeine in energy beverages was developed and validated. Sample preparation utilizing solid-phase extraction (SPE) was simple and reliable. Separation by isocratic ultra-high performance liquid chromatography (UPLC) with a reversed-phase C18 column was performed within 6 min. The use of SPE with UPLC coupled with electrospray ionization-multiple tandem mass spectrometry detection (ESI-MSn) was accurate, reproducible, and validated for the determination of caffeine in energy drink matrices. The limit of quantification for caffeine was approximately 2.1 ng mL-1. The intra-day and inter-day precisions were less than 4%, and the accuracy of the measurements was between 85.1% and 93.2%. Results for caffeine concentrations in eighteen beverages were compared to the values on the labels. This paper describes the first use of the UPLC-ESI-ion trap MSn technique for quality-control purposes of caffeine present in energy drinks.

AB - A simple and highly sensitive method for the determination of caffeine in energy beverages was developed and validated. Sample preparation utilizing solid-phase extraction (SPE) was simple and reliable. Separation by isocratic ultra-high performance liquid chromatography (UPLC) with a reversed-phase C18 column was performed within 6 min. The use of SPE with UPLC coupled with electrospray ionization-multiple tandem mass spectrometry detection (ESI-MSn) was accurate, reproducible, and validated for the determination of caffeine in energy drink matrices. The limit of quantification for caffeine was approximately 2.1 ng mL-1. The intra-day and inter-day precisions were less than 4%, and the accuracy of the measurements was between 85.1% and 93.2%. Results for caffeine concentrations in eighteen beverages were compared to the values on the labels. This paper describes the first use of the UPLC-ESI-ion trap MSn technique for quality-control purposes of caffeine present in energy drinks.

KW - Caffeine

KW - Energy drinks

KW - HPLC-ESI-MS/MS

KW - Solid-phase extraction

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DO - 10.1080/00032719.2014.888730

M3 - Article

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SN - 0003-2719

VL - 47

SP - 1852

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JO - Analytical Letters

JF - Analytical Letters

IS - 11

ER -

Ultra-High Performance Liquid Chromatography with Electrospray Ionization Tandem Mass Spectrometry for the Determination of Caffeine in Energy Drinks

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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