Ultra-specific enrichment of gst-tagged protein by gsh-modified nanoparticles

Yeon Ji Lee; Jong Moon Park; Jiyoung Huh; Minsik Kim; Je Sun Lee; Arudra Palani; Kwangyeol Lee; Sang Won Lee

doi:10.5012/bkcs.2010.31.6.1568

Ultra-specific enrichment of gst-tagged protein by gsh-modified nanoparticles

Yeon Ji Lee^*
, Jong Moon Park
, Jiyoung Huh
, Minsik Kim
, Je Sun Lee
, Arudra Palani
, Kwangyeol Lee
, Sang Won Lee

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathione- modified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/ MS/MS experiments.

Original language	English
Pages (from-to)	1568-1572
Number of pages	5
Journal	Bulletin of the Korean Chemical Society
Volume	31
Issue number	6
DOIs	https://doi.org/10.5012/bkcs.2010.31.6.1568
Publication status	Published - 2010 Jun 20

Keywords

Glutathione
Nanoparticle
Proteomics
Separation
Superparamagnetic

ASJC Scopus subject areas

General Chemistry

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10.5012/bkcs.2010.31.6.1568

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abstract = "The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathione- modified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/ MS/MS experiments.",

keywords = "Glutathione, Nanoparticle, Proteomics, Separation, Superparamagnetic",

author = "Lee, \{Yeon Ji\} and Park, \{Jong Moon\} and Jiyoung Huh and Minsik Kim and Lee, \{Je Sun\} and Arudra Palani and Kwangyeol Lee and Lee, \{Sang Won\}",

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language = "English",

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T1 - Ultra-specific enrichment of gst-tagged protein by gsh-modified nanoparticles

AU - Lee, Yeon Ji

AU - Park, Jong Moon

AU - Huh, Jiyoung

AU - Kim, Minsik

AU - Lee, Je Sun

AU - Palani, Arudra

AU - Lee, Kwangyeol

AU - Lee, Sang Won

PY - 2010/6/20

Y1 - 2010/6/20

N2 - The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathione- modified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/ MS/MS experiments.

AB - The selective isolation of specific proteins from complex protein mixtures by nanoparticles is reported. Glutathione- modified superparamagnetic nanoparticles were used to purify specific proteins fused with glutathione S-transferase via enzyme-substrate interactions. They demonstrated greatly improved selectivity and efficiency over micron sized capturing beads. The ultra-specific enrichment of target proteins was confirmed by both SDS-PAGE and LC/ MS/MS experiments.

KW - Glutathione

KW - Nanoparticle

KW - Proteomics

KW - Separation

KW - Superparamagnetic

UR - https://www.scopus.com/pages/publications/77953936753

U2 - 10.5012/bkcs.2010.31.6.1568

DO - 10.5012/bkcs.2010.31.6.1568

M3 - Article

AN - SCOPUS:77953936753

SN - 0253-2964

VL - 31

SP - 1568

EP - 1572

JO - Bulletin of the Korean Chemical Society

JF - Bulletin of the Korean Chemical Society

IS - 6

ER -

Ultra-specific enrichment of gst-tagged protein by gsh-modified nanoparticles

Abstract

Keywords

ASJC Scopus subject areas

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