Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex

Inhong Kim; Kwangseuk Kyhm; Mijeong Kang; Han Young Woo

doi:10.1016/j.jlumin.2014.01.042

Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex

Inhong Kim, Kwangseuk Kyhm, Mijeong Kang, Han Young Woo

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The combined dynamic process of Förster resonance energy transfer and transient quenching is quantified in the time-resolved fluorescence of cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. We found that the radiation boundary condition fails to predict transient quenching due to a single quenching rate at the encounter distance between a fluorophore and a quencher; however, the predictions of the micellar kinetics model were in good agreement with the measured time-resolved fluorescence as an alternative to the complicated distance-dependent quenching model. The combined dynamics model enables the separation of the rate of Förster resonance energy transfer from that of transient quenching, by which we obtained an accurate estimation of the donor-acceptor intermolecular distance (41±1.6 Å) in comparison with the Förster distance (43 Å).

Original language	English
Pages (from-to)	185-189
Number of pages	5
Journal	Journal of Luminescence
Volume	149
DOIs	https://doi.org/10.1016/j.jlumin.2014.01.042
Publication status	Published - 2014 May
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by Ministry of Education, Science and Technology ( BRL2011-0001198 and 2012R1A1A2006913 ), and Pioneer Research Center (NRF-2013M3C1A3065522).

Keywords

Conjugated polymer
Exciton
Fluorescence quenching model
Förster resonance energy transfer (FRET)
Time-resolved fluorescence

ASJC Scopus subject areas

Biophysics
Biochemistry
General Chemistry
Atomic and Molecular Physics, and Optics
Condensed Matter Physics

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10.1016/j.jlumin.2014.01.042

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@article{4ac96f3b697143df80475e7250be5f0c,

title = "Ultrafast combined dynamics of F{\"o}rster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex",

abstract = "The combined dynamic process of F{\"o}rster resonance energy transfer and transient quenching is quantified in the time-resolved fluorescence of cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. We found that the radiation boundary condition fails to predict transient quenching due to a single quenching rate at the encounter distance between a fluorophore and a quencher; however, the predictions of the micellar kinetics model were in good agreement with the measured time-resolved fluorescence as an alternative to the complicated distance-dependent quenching model. The combined dynamics model enables the separation of the rate of F{\"o}rster resonance energy transfer from that of transient quenching, by which we obtained an accurate estimation of the donor-acceptor intermolecular distance (41±1.6 {\AA}) in comparison with the F{\"o}rster distance (43 {\AA}).",

keywords = "Conjugated polymer, Exciton, Fluorescence quenching model, F{\"o}rster resonance energy transfer (FRET), Time-resolved fluorescence",

author = "Inhong Kim and Kwangseuk Kyhm and Mijeong Kang and Woo, {Han Young}",

note = "Funding Information: This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by Ministry of Education, Science and Technology ( BRL2011-0001198 and 2012R1A1A2006913 ), and Pioneer Research Center (NRF-2013M3C1A3065522).",

year = "2014",

month = may,

doi = "10.1016/j.jlumin.2014.01.042",

language = "English",

volume = "149",

pages = "185--189",

journal = "Journal of Luminescence",

issn = "0022-2313",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex

AU - Kim, Inhong

AU - Kyhm, Kwangseuk

AU - Kang, Mijeong

AU - Woo, Han Young

N1 - Funding Information: This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by Ministry of Education, Science and Technology ( BRL2011-0001198 and 2012R1A1A2006913 ), and Pioneer Research Center (NRF-2013M3C1A3065522).

PY - 2014/5

Y1 - 2014/5

N2 - The combined dynamic process of Förster resonance energy transfer and transient quenching is quantified in the time-resolved fluorescence of cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. We found that the radiation boundary condition fails to predict transient quenching due to a single quenching rate at the encounter distance between a fluorophore and a quencher; however, the predictions of the micellar kinetics model were in good agreement with the measured time-resolved fluorescence as an alternative to the complicated distance-dependent quenching model. The combined dynamics model enables the separation of the rate of Förster resonance energy transfer from that of transient quenching, by which we obtained an accurate estimation of the donor-acceptor intermolecular distance (41±1.6 Å) in comparison with the Förster distance (43 Å).

AB - The combined dynamic process of Förster resonance energy transfer and transient quenching is quantified in the time-resolved fluorescence of cationic polyfluorene/fluorescein-labelled single-stranded DNA complex. We found that the radiation boundary condition fails to predict transient quenching due to a single quenching rate at the encounter distance between a fluorophore and a quencher; however, the predictions of the micellar kinetics model were in good agreement with the measured time-resolved fluorescence as an alternative to the complicated distance-dependent quenching model. The combined dynamics model enables the separation of the rate of Förster resonance energy transfer from that of transient quenching, by which we obtained an accurate estimation of the donor-acceptor intermolecular distance (41±1.6 Å) in comparison with the Förster distance (43 Å).

KW - Conjugated polymer

KW - Exciton

KW - Fluorescence quenching model

KW - Förster resonance energy transfer (FRET)

KW - Time-resolved fluorescence

UR - http://www.scopus.com/inward/record.url?scp=84893504426&partnerID=8YFLogxK

U2 - 10.1016/j.jlumin.2014.01.042

DO - 10.1016/j.jlumin.2014.01.042

M3 - Article

AN - SCOPUS:84893504426

SN - 0022-2313

VL - 149

SP - 185

EP - 189

JO - Journal of Luminescence

JF - Journal of Luminescence

ER -

Ultrafast combined dynamics of Förster resonance energy transfer and transient quenching in cationic polyfluorene/fluorescein-labelled single-stranded DNA complex

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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