Undefined xylose media extracted from biorefinery waste for enhanced and eco-friendly production of cephalosporin C by Acremonium chrysogenum M35

Sungbong Kim, Hyeong Ryeol Kim, Hyun Ju Oh, Kyu Tae Lee, Youngsoon Um, Dong Wook Kim, Sang Woo Seo, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review


Xylose-rich undefined broth, extracted from the dilute acid pretreatment wastes of barley straw, serves as resourceful media for Acremonium chrysogenum M35 culture and production of cephalosporin C (CPC). Concentrating the extract with proper reprocessing enables to prepare various concentrations of xylose broth (2%–8%). The undefined xylose media were prepared for CPC production from A. chrysogenum M35 by the addition of other nutrients. Cell growth and CPC production were the most effective at 6% xylose and additional 2% glycerol, with maximum CPC production of 9.07 g/L after 6 days, which is higher production than that in defined media prepared with laboratory-level nutrients and reagents. Investigation of autotrophic and reverse trans-sulfuration pathways for cysteine synthesis, a limited element of three precursors for CPC synthesis, supports the enhanced CPC production in undefined media. Abundance of xylose ensures a maintained NADPH concentration required for sulfate reduction and synthesis of amino sulfide such as cysteine. Cystathionine-γ-lyase activity profiling indicated more efficient biosynthesis in undefined media than in other cultures use glycerol and glucose, and the biosynthesis pathway of CPC production by the cephalosporin gene cluster (i.e. pcbC and cefG genes) was investigated. The process using undefined xylose media was designed, and process simulation program confirmed our results.

Original languageEnglish
Pages (from-to)731-742
Number of pages12
JournalGCB Bioenergy
Issue number5
Publication statusPublished - 2023 May

Bibliographical note

Funding Information:
This study was supported by the Basic Researcher Program of the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (MSIT) [NRF‐2022R1F1A1068710], the Bio and Medical Technology Development Program of the NRF funded by the MSIT [NRF‐2018M3A9H3020459] and the research grant of Kwangwoon University (2022).

Publisher Copyright:
© 2023 The Authors. GCB Bioenergy published by John Wiley & Sons Ltd.


  • biorefinery
  • cephalosporin C
  • dilute acid pretreatment
  • echo-friendly process
  • waste reprocess
  • xylose fermentation

ASJC Scopus subject areas

  • Forestry
  • Renewable Energy, Sustainability and the Environment
  • Agronomy and Crop Science
  • Waste Management and Disposal


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