Surface-enhanced Raman scattering (SERS) is a promising technique for molecular analysis as the molecular fingerprints (Raman spectra) are amplified to detectable levels compared with common spectroscopy. Metal nanostructures localize electromagnetic field on their surfaces, which can lead to dramatic increase of Raman intensity of molecules adsorbed. However, the metal surfaces are prone to contamination, thereby requiring pretreatment of samples to remove adhesive molecules. To avoid the pretreatment and potentially achieve point-of-care (POC) analysis, we have developed SERS-active microgels using the droplet-microfluidic system. As the microgels are composed of water-swollen network with consistent mesh size, they selectively allow diffusion of molecules smaller than the mesh, thereby excluding large adhesives. To render the microgels highly SERS-active, we destabilize silver nanocubes to form agglomerates, which are embedded in the matrix of microgels. The nanogaps in the agglomerates provide high sensitivity in Raman measurement and size-selective permeability of the microgel matrix obviates the pretreatment of samples. To validate the functions, we demonstrate the direct detection of Aspirin dissolved in whole blood without any pretreatment.
Bibliographical noteFunding Information:
This work was supported by the Fundamental Research Program (PNK 4660) of the Korean Institute of Materials Science (KIMS) and the Midcareer Researcher Program (2014R1A2A2A01005813) and Global Research Laboratory (NRF-2015K1A1A2033054) through the National Research Foundation (NRF) grant funded by the Ministry of Science, ICT and Future Planning (MSIP). The authors thank Prof. Jaemoon Yang at Yonsei University for providing rat blood.
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
- surface-enhanced Raman scattering
ASJC Scopus subject areas
- Engineering (miscellaneous)
- Materials Science(all)