Uniform-sized neurosphere-mediated motoneuron differentiation in microwell arrays

Jong Min Lee; Joo Yoon Moon; Mohammed R. Shaker; Woong Sun; Bong Geun Chung

doi:10.1002/elps.201700118

Uniform-sized neurosphere-mediated motoneuron differentiation in microwell arrays

Jong Min Lee, Joo Yoon Moon, Mohammed R. Shaker, Woong Sun, Bong Geun Chung

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

We developed the photocrosslinkable hydrogel microwell arrays for uniform-sized neurosphere-mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform-sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform-sized neurospheres harvested from microwell arrays were replated into laminin-coated substrate. In parallel, uniform-sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three-dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform-sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform-sized neurosphere-mediated motoneuron differentiation.

Original language	English
Pages (from-to)	3161-3167
Number of pages	7
Journal	Electrophoresis
Volume	38
Issue number	24
DOIs	https://doi.org/10.1002/elps.201700118
Publication status	Published - 2017 Dec

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation (NRF) of Korea grant funded by the Ministry of Science, ICT & Future Planning (MSIP) (Grant number 2016R1A6A1A03012845). This research was supported by C1 Gas Refinery Program through the NRF of Korea funded by the MSIP (Grant number 2015M3D3A1A01064926). This work was also supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health & Welfare, Republic of Korea (Grant number HI14C3347).

Publisher Copyright:
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

Hydrogel microwell array
Motoneuron differentiation
Uniform-sized neurosphere

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry

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10.1002/elps.201700118

Cite this

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title = "Uniform-sized neurosphere-mediated motoneuron differentiation in microwell arrays",

abstract = "We developed the photocrosslinkable hydrogel microwell arrays for uniform-sized neurosphere-mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform-sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform-sized neurospheres harvested from microwell arrays were replated into laminin-coated substrate. In parallel, uniform-sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three-dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform-sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform-sized neurosphere-mediated motoneuron differentiation.",

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author = "Lee, {Jong Min} and Moon, {Joo Yoon} and Shaker, {Mohammed R.} and Woong Sun and Chung, {Bong Geun}",

note = "Funding Information: This work was supported by the National Research Foundation (NRF) of Korea grant funded by the Ministry of Science, ICT & Future Planning (MSIP) (Grant number 2016R1A6A1A03012845). This research was supported by C1 Gas Refinery Program through the NRF of Korea funded by the MSIP (Grant number 2015M3D3A1A01064926). This work was also supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute, funded by the Ministry of Health & Welfare, Republic of Korea (Grant number HI14C3347). Publisher Copyright: {\textcopyright} 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

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N2 - We developed the photocrosslinkable hydrogel microwell arrays for uniform-sized neurosphere-mediated motoneuron differentiation. Neural stem cells (NSCs) were obtained from embryonic cerebral cortex and spinal cord. To generate uniform-sized neurospheres in a homogeneous manner, the dissociated cells were cultured in the hydrogel microwell arrays for 3 days. Uniform-sized neurospheres harvested from microwell arrays were replated into laminin-coated substrate. In parallel, uniform-sized neurospheres cultured in microwell arrays were encapsulated by photocrosslinkable gelatin methacrylate hydrogels in a three-dimensional manner. We demonstrated the effect of hydrogel microwell sizes (e.g., 50, 100, 150 μm in diameter) on motoneuron differentiation, showing that the largest uniform-sized neurospheres derived from embryonic spinal cord efficiently differentiated into motoneurons. Therefore, this hydrogel microwell array could be a powerful array to regulate the uniform-sized neurosphere-mediated motoneuron differentiation.

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Uniform-sized neurosphere-mediated motoneuron differentiation in microwell arrays

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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