TY - JOUR
T1 - Urechidicola croceus gen. nov., sp. nov., a member of the family Flavobacteriaceae
AU - Shin, Su Kyoung
AU - Yi, Hana
N1 - Funding Information:
This study was supported by the Survey of Korean Indigenous Species Program through the National Institute of Biological Resources funded by the Korean Ministry of Environment and by a National Research Council of Science and Technology grant from the Korean government (MSIP) (No. CRC-16–01-KRICT).
Publisher Copyright:
© 2020 The Authors.
PY - 2020
Y1 - 2020
N2 - A Gram-stain-negative, rod-shaped, non-flagellated, non-gliding, aerobic bacterial strain, designated LPB0138T, was isolated from a marine spoon worm (Urechis unicinctus). The strain LPB0138T contains a circular chromosome of 3.43 Mb with a DNA G+C content of 30.4 mol%. The genome includes 2987 protein-coding genes and two copies of rRNA operons. The 16S rRNA gene sequence analysis showed that the isolate occurred within a clade containing only members of the family Flavobacte-riaceae. The highest sequence similarity was observed with the genus Lutibacter (93.0–94.3%), but the phylogenetic leaf of the new isolate did not belong to any of the genera known in the family Flavobacteriaceae. The low sequence similarity and the phylogenetic tree topology implied the novel generic status of the new isolate. The phenotypic properties of the strain LPB0138T also differentiated this isolate from its neighbour genera by showing a distinctive fatty acid composition, unique polar lipids profile, and low DNA G+C content. The LPB0138T strain contained menaquinone 6 as the isoprenoid quinone; iso-C15:1 G, iso-C15:0, iso-C15:0 3-OH, and iso-C17:0 3-OH as the major fatty acids; and phosphatidylethanolamine, unidentified aminophospholipids, unidentified aminolipids, and unidentified lipids as the major polar lipids. Based on the polyphasic taxonomic data obtained, the LPB0138T strain is considered to represent a novel species in a novel genus of the family Flavobacteriaceae, for which the name Urechidicola croceus gen. nov., sp. nov. was proposed. The type strain is LPB0138T (=KACC 18889T;=JCM 31563T).
AB - A Gram-stain-negative, rod-shaped, non-flagellated, non-gliding, aerobic bacterial strain, designated LPB0138T, was isolated from a marine spoon worm (Urechis unicinctus). The strain LPB0138T contains a circular chromosome of 3.43 Mb with a DNA G+C content of 30.4 mol%. The genome includes 2987 protein-coding genes and two copies of rRNA operons. The 16S rRNA gene sequence analysis showed that the isolate occurred within a clade containing only members of the family Flavobacte-riaceae. The highest sequence similarity was observed with the genus Lutibacter (93.0–94.3%), but the phylogenetic leaf of the new isolate did not belong to any of the genera known in the family Flavobacteriaceae. The low sequence similarity and the phylogenetic tree topology implied the novel generic status of the new isolate. The phenotypic properties of the strain LPB0138T also differentiated this isolate from its neighbour genera by showing a distinctive fatty acid composition, unique polar lipids profile, and low DNA G+C content. The LPB0138T strain contained menaquinone 6 as the isoprenoid quinone; iso-C15:1 G, iso-C15:0, iso-C15:0 3-OH, and iso-C17:0 3-OH as the major fatty acids; and phosphatidylethanolamine, unidentified aminophospholipids, unidentified aminolipids, and unidentified lipids as the major polar lipids. Based on the polyphasic taxonomic data obtained, the LPB0138T strain is considered to represent a novel species in a novel genus of the family Flavobacteriaceae, for which the name Urechidicola croceus gen. nov., sp. nov. was proposed. The type strain is LPB0138T (=KACC 18889T;=JCM 31563T).
KW - Flavobacteriaceae
KW - Spoon worm
KW - Urechidicola
KW - Urechis unicinctus
UR - http://www.scopus.com/inward/record.url?scp=85082779806&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.003966
DO - 10.1099/ijsem.0.003966
M3 - Article
C2 - 31935176
AN - SCOPUS:85082779806
SN - 1466-5026
VL - 70
SP - 1751
EP - 1757
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 3
M1 - 003966
ER -
