Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

Hyun Yong Shin; Jin Young Lee; Chulhwan Park; Seung Wook Kim

doi:10.1016/j.jbiotec.2010.12.021

Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

Hyun Yong Shin, Jin Young Lee, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

In this study, the role of glycerol in cephalosporin C production was evaluated in Acremonium chrysogenum M35. Methionine-unsupplemented culture with glycerol stimulated cephalosporin C production in A. chrysogenum M35 by up to 10-fold while increasing the amount of cysteine. Glycerol caused the upregulation of isopenicillin N-CoA epimerase (cefD2) and δ-(l-α-aminoadipyl)-l-cysteinyl-D-valine synthase (pcbAB) transcription in exponential phase. These results indicate that glycerol without methionine effectively enhanced cephalosporin C production via stimulation of cysteine and valine in A. chrysogemun M35.

Original language	English
Pages (from-to)	363-368
Number of pages	6
Journal	Journal of Biotechnology
Volume	151
Issue number	4
DOIs	https://doi.org/10.1016/j.jbiotec.2010.12.021
Publication status	Published - 2011 Feb 20

Keywords

Acremonium chrysogenum
Cephalosporin C
Cysteine
Methionine

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1016/j.jbiotec.2010.12.021

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title = "Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture",

abstract = "In this study, the role of glycerol in cephalosporin C production was evaluated in Acremonium chrysogenum M35. Methionine-unsupplemented culture with glycerol stimulated cephalosporin C production in A. chrysogenum M35 by up to 10-fold while increasing the amount of cysteine. Glycerol caused the upregulation of isopenicillin N-CoA epimerase (cefD2) and δ-(l-α-aminoadipyl)-l-cysteinyl-D-valine synthase (pcbAB) transcription in exponential phase. These results indicate that glycerol without methionine effectively enhanced cephalosporin C production via stimulation of cysteine and valine in A. chrysogemun M35.",

keywords = "Acremonium chrysogenum, Cephalosporin C, Cysteine, Methionine",

author = "Shin, {Hyun Yong} and Lee, {Jin Young} and Chulhwan Park and Kim, {Seung Wook}",

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T1 - Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

AU - Shin, Hyun Yong

AU - Lee, Jin Young

AU - Park, Chulhwan

AU - Kim, Seung Wook

PY - 2011/2/20

Y1 - 2011/2/20

N2 - In this study, the role of glycerol in cephalosporin C production was evaluated in Acremonium chrysogenum M35. Methionine-unsupplemented culture with glycerol stimulated cephalosporin C production in A. chrysogenum M35 by up to 10-fold while increasing the amount of cysteine. Glycerol caused the upregulation of isopenicillin N-CoA epimerase (cefD2) and δ-(l-α-aminoadipyl)-l-cysteinyl-D-valine synthase (pcbAB) transcription in exponential phase. These results indicate that glycerol without methionine effectively enhanced cephalosporin C production via stimulation of cysteine and valine in A. chrysogemun M35.

AB - In this study, the role of glycerol in cephalosporin C production was evaluated in Acremonium chrysogenum M35. Methionine-unsupplemented culture with glycerol stimulated cephalosporin C production in A. chrysogenum M35 by up to 10-fold while increasing the amount of cysteine. Glycerol caused the upregulation of isopenicillin N-CoA epimerase (cefD2) and δ-(l-α-aminoadipyl)-l-cysteinyl-D-valine synthase (pcbAB) transcription in exponential phase. These results indicate that glycerol without methionine effectively enhanced cephalosporin C production via stimulation of cysteine and valine in A. chrysogemun M35.

KW - Acremonium chrysogenum

KW - Cephalosporin C

KW - Cysteine

KW - Methionine

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DO - 10.1016/j.jbiotec.2010.12.021

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Utilization of glycerol as cysteine and carbon sources for cephalosporin C production by Acremonium chrysogenum M35 in methionine-unsupplemented culture

Abstract

Keywords

ASJC Scopus subject areas

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