Abstract
A highly sensitive analytical tool for the fast quantification of irsogladine in human plasma was developed. Cleanup using a solid-phase extraction technique is a simple method for extracting both irsogladine and lamotrigine (internal standard) spiked into human plasma. The resolvable separation of both analytes through reversed-phase high-performance liquid chromatography (HPLC) was carried out within 5 min. The HPLC-electrospray ionization (ESI)-tandem mass spectrometry (MS/MS) method, which was operated in a selected reaction monitoring mode specific to the target analytes, was verified for use in the quantification of irsogladine. The inter- and intra-day precision (relative standard deviation, RSD) of irsogladine spiked into quality control samples were <7%, and their accuracies were between 96.6% and 102.1%. The calibration curve for irsogladine spiked into human plasma was linear over the range from 1.8 to 100 ng mL−1 with lower limit of quantification at 1.8 ng mL−1. The established method was successfully applied for a bioequivalence study of irsogladine.
Original language | English |
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Pages (from-to) | 459-462 |
Number of pages | 4 |
Journal | Acta Chromatographica |
Volume | 29 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2017 Dec |
Bibliographical note
Publisher Copyright:© 2017 The Author(s)
Keywords
- Bioequivalence study
- HPLC-ESI-MS/MS
- Human plasma
- Irsogladine
ASJC Scopus subject areas
- General Chemistry