Validation of the metabolic pathway of the alginate-derived monomer in Saccharophagus degradans 2-40T by gas chromatography–mass spectrometry

Do Hyoung Kim, Damao Wang, Eun Ju Yun, Sooah Kim, Soo Rin Kim, Kyoung Heon Kim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Marine macroalgae are potential resources for the sustainable production of biofuels and bio-based chemicals. Alginate, a major component of brown macroalgae, consists of two uronate monomers, which are further non-enzymatically converted to 4-deoxy-L-erythro-5-hexoseulose uronate (DEH). In several marine bacteria, DEH is known to be metabolized via three enzymatic steps, consisting of DEH reductase, 2-keto-3-deoxy-D-gluconate (KDG) kinase, and 2-keto-3-deoxy-phosphogluconate (KDPG) aldolase, which yields two glycolytic intermediates: D-glyceraldehyde-3-phosphate and pyruvate. However, such functions of these enzymes for the DEH pathway have rarely been experimentally validated. In the present study, the DEH metabolic pathway was investigated in Saccharophagus degradans 2-40T, a marine bacterium that utilizes alginate. Through in vitro tests assisted by gas chromatography/mass spectrometry and gas chromatography/time-of-flight mass spectrometry, the purified enzymes were functionally confirmed and annotated as dehR, kdgK, and kdpgA, respectively. In conclusion, we report the in vitro validation of the metabolic pathway of DEH monomerized from alginate.

Original languageEnglish
Pages (from-to)1374-1379
Number of pages6
JournalProcess Biochemistry
Issue number10
Publication statusPublished - 2016 Oct 1

Bibliographical note

Funding Information:
This work was supported by a grant from the National Research Foundation of Korea ( 2013M1A2A2072597 ), funded by the Korean Government (MSIP). The study was performed at the Korea University Food Safety Hall for the Institute of Biomedical Science and Food Safety.

Publisher Copyright:
© 2016 Elsevier Ltd


  • 2-Keto-3-deoxy-D-gluconate
  • 2-Keto-3-deoxy-phosphogluconate
  • 4-Deoxy-L-erythro-5-hexoseulose uronate
  • Alginate metabolism
  • Saccharophagus degradans 2-40
  • Short-chain dehydrogenase/reductase

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology


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