TY - JOUR
T1 - Variation in pathogenicity of a mountain pine beetle-associated blue-stain fungus, Grosmannia clavigera, on young lodgepole pine in British Columbia
AU - Plattner, Alex
AU - Kim, Jae Jin
AU - DiGuistini, Scott
AU - Breuil, Colette
PY - 2008/9
Y1 - 2008/9
N2 - Grosmannia clavigera is the most pathogenic blue-staining fungal associate of the mountain pine beetle (Dendroctonus ponderosae). In contrast to its importance as a primary invader of lodgepole pine (Pinus contorta) sapwood, intraspecific variability in pathogenicity of G. clavigera on lodgepole pine, the predominant host of mountain pine beetles in British Columbia, has not been investigated in detail. The present work reports on pathogenicity indicators induced by five G. clavigera isolates inoculated into lodgepole pines and growth characteristics of the isolates on artificial media. Fungi were inoculated at 200 inoculations/m2 into young lodgepole pine trees. Phloem lesion length, sapwood occlusion area, and sapwood moisture content were measured after 7 or 48 weeks. Three isolates produced long lesions, occluded larger areas, and reduced more the moisture content after 48 weeks compared with the remaining two isolates. Isolate ATCC 18086 induced the strongest pathogenic symptoms after 7 weeks and grew the fastest up to 22.5 °C but grew the slowest at 27.5 °C. In a low-oxygen environment, most isolates grew faster than under ambient conditions. Significant intraspecific variation was observed among G. clavigera isolates for all parameters tested.
AB - Grosmannia clavigera is the most pathogenic blue-staining fungal associate of the mountain pine beetle (Dendroctonus ponderosae). In contrast to its importance as a primary invader of lodgepole pine (Pinus contorta) sapwood, intraspecific variability in pathogenicity of G. clavigera on lodgepole pine, the predominant host of mountain pine beetles in British Columbia, has not been investigated in detail. The present work reports on pathogenicity indicators induced by five G. clavigera isolates inoculated into lodgepole pines and growth characteristics of the isolates on artificial media. Fungi were inoculated at 200 inoculations/m2 into young lodgepole pine trees. Phloem lesion length, sapwood occlusion area, and sapwood moisture content were measured after 7 or 48 weeks. Three isolates produced long lesions, occluded larger areas, and reduced more the moisture content after 48 weeks compared with the remaining two isolates. Isolate ATCC 18086 induced the strongest pathogenic symptoms after 7 weeks and grew the fastest up to 22.5 °C but grew the slowest at 27.5 °C. In a low-oxygen environment, most isolates grew faster than under ambient conditions. Significant intraspecific variation was observed among G. clavigera isolates for all parameters tested.
KW - Blue-stain fungus
KW - Grosmannia clavigera
KW - Intraspecific variation
KW - Mountain pine beetle
KW - Ophiostoma clavigerum
KW - Pathogenicity
UR - http://www.scopus.com/inward/record.url?scp=77953232153&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953232153&partnerID=8YFLogxK
U2 - 10.1080/07060660809507543
DO - 10.1080/07060660809507543
M3 - Article
AN - SCOPUS:77953232153
SN - 0706-0661
VL - 30
SP - 457
EP - 466
JO - Canadian Journal of Plant Pathology
JF - Canadian Journal of Plant Pathology
IS - 3
ER -