Various clinical application of phase contrast x-ray

Chil Hwan Oh, Sangyong Park, Seunghan Ha, Gyuman Park, Gunwoo Lee, Onseok Lee, Jungho Je

    Research output: Chapter in Book/Report/Conference proceedingConference contribution


    In biomedical application study using phase contrast X-ray, both sample thickness or density and absorption difference are very important factors in aspects of contrast enhancement. We present experimental evidence that synchrotron hard X-ray are suitable for radiological imaging of biological samples down to the cellular level. We investigated the potential of refractive index radiology using un-monochromatized synchrotron hard X-rays for the imaging of cell and tissue in various diseases. Material had been adopted various medical field, such as apoE knockout mouse in cardiologic field, specimen from renal and prostatic carcinoma patient in urology, basal cell epithelioma in dermatology, brain tissue from autosy sample of pakinson's disease, artificially induced artilrtis tissue from rabbits and extracted tooth from patients of crack tooth syndrome. Formalin and paraffin fixed tissue blocks were cut in 3 mm thickness for the X-ray radiographic imaging. From adjacent areas, 4 μm thickness sections were also prepared for hematoxylin-eosin staining. Radiographic images of dissected tissues were obtained using the hard X-rays from the 7B2 beamline of the Pohang Light Source (PLS). The technique used for the study was the phase contrast images were compared with the optical microscopic images of corresponding histological slides. Radiographic images of various diseased tissues showed clear histological details of organelles in normal tissues. Most of cancerous lesions were well differentiated from adjacent normal tissues and detailed histological features of each tumor were clearly identified. Also normal microstructures were identifiable by the phase contrast imaging. Tissue in cancer or other disease showed clearly different findings from those of surrounding normal tissue. For the first time we successfully demonstrated that synchrotron hard X-rays can be used for radiological imaging of relatively thick tissue samples with great histological details.

    Original languageEnglish
    Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems VI
    Publication statusPublished - 2008 Apr 21
    EventAdvanced Biomedical and Clinical Diagnostic Systems VI - San Jose, CA, United States
    Duration: 2008 Jan 202008 Jan 21


    OtherAdvanced Biomedical and Clinical Diagnostic Systems VI
    Country/TerritoryUnited States
    CitySan Jose, CA


    • Clinical application
    • Phase contrast X-ray

    ASJC Scopus subject areas

    • General Engineering


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