Vascular reactivity contributes to adipose tissue remodeling in obesity

Hye Jin Lee, Haifei Shi, Hella S. Brönneke, Bo Yeong Jin, Sang Hyun Choi, Randy J. Seeley, Dong Hoon Kim

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Vascular reactivity of adipose tissue (AT) is hypothesized to play an important role in the development of obesity. However, the exact role of vascular reactivity in the development of obesity remains unclear. In this study, we investigated the chronological changes in vascular reactivity and the microenvironments of the visceral AT (VAT) and subcutaneous AT (SAT) in lean and obese mice. Changes in blood flow levels induced by a β-adrenoceptor agonist (isoproterenol) were significantly lower in the VAT of the mice fed a high-fat diet (HFD) for 1 and 12 weeks than those in the VAT of the mice fed a low-fat diet (LFD) for the same period; no significant change was observed in the SAT of any mouse group, suggesting depot-specific vascular reactivity of AT. Moreover, the hypoxic area and the expression of genes associated with angiogenesis and macrophage recruitment were increased in the VAT (but not in the SAT) of mice fed an HFD for 1 week compared with mice fed an LFD. These changes occurred with no morphological changes, including those related to adipocyte size, AT vessel density, and the diameter and pericyte coverage of the endothelium, suggesting a determinant role of vascular reactivity in the type of AT remodeling. The suppression of vascular reactivity was accompanied by increased endothelin1 (Edn1) gene expression and extracellular matrix (ECM) stiffness only in the VAT, implying enhanced contractile activities of the vasculature and ECM. The results suggest a depot-specific role of vascular reactivity in AT remodeling during the development of obesity.

Original languageEnglish
Pages (from-to)195-206
Number of pages12
JournalJournal of Endocrinology
Volume251
Issue number3
DOIs
Publication statusPublished - 2021 Dec

Bibliographical note

Funding Information:
This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), which is funded by the Ministry of Health & Welfare, Republic of Korea (Grant No. HI12C1541) and a Korea University Grant. This work was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (Grant No. 2017R1D1A1B03031216) and the Bio & Medical Technology Development Program through the NRF funded by the Ministry of Science & ICT (Grant No. 2015M3A9E7029172). The authors would like to thank the Institute of Biomedical Science and Food Safety at the Korea University Food Safety Hall (Seoul, Korea) for technical support.

Funding Information:
This work was supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), which is funded by the Ministry of Health & Welfare, Republic of Korea (Grant No. HI12C1541) and a Korea University Grant. This work was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (Grant No. 2017R1D1A1B03031216) and the Bio & Medical Technology Development Program through the NRF funded by the Ministry of Science & ICT (Grant No. 2015M3A9E7029172).

Publisher Copyright:
© 2021 Society for Endocrinology.

Keywords

  • adipose tissue blood flow
  • adipose tissue remodeling
  • beta-adrenergic receptor
  • hypoxia
  • obesity

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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