Vibrio vulnificus secretes an Insulin-degrading enzyme that Promotes Bacterial Proliferation in Vivo

In Hwang Kim, Ik Jung Kim, Yancheng Wen, Na Young Park, Jinyoung Park, Keun Woo Lee, Ara Koh, Ji Hyun Lee, Seung Hoi Koo, Kun Soo Kim

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


We describe a novel insulin-degrading enzyme, SidC, that contributes to the proliferation of the human bacterial pathogen Vibrio vulnificus in a mouse model. SidC is phylogenetically distinct from other known insulin-degrading enzymes and is expressed and secreted specifically during host infection. Purified SidC causes a significant decrease in serum insulin levels and an increase in blood glucose levels in mice. A comparison of mice infected with wild type V. vulnificus or an isogenic sidC-deletion strain showed that wild type bacteria proliferated to higher levels. Additionally, hyperglycemia leads to increased proliferation of V. vulnificus in diabetic mice. Consistent with these observations, the sid operon was up-regulated in response to low glucose levels through binding of the cAMP-receptor protein (CRP) complex to a region upstream of the operon. We conclude that glucose levels are important for the survival of V. vulnificus in the host, and that this pathogen uses SidC to actively manipulate host endocrine signals, making the host environment more favorable for bacterial survival and growth.

Original languageEnglish
Pages (from-to)18708-18720
Number of pages13
JournalJournal of Biological Chemistry
Issue number30
Publication statusPublished - 2015 Jul 24

Bibliographical note

Publisher Copyright:
© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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