Vimentin filaments regulate integrin-ligand interactions by binding to the cytoplasmic tail of integrin β3

Jiyoon Kim, Chansik Yang, Eun Jin Kim, Jungim Jang, Se Jong Kim, So Min Kang, Moon Gyo Kim, Hosung Jung, Dongeun Park, Chungho Kim

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Vimentin, an intermediate filament protein induced during epithelialto- mesenchymal transition, is known to regulate cell migration and invasion. However, it is still unclear how vimentin controls such behaviors. In this study, we aimed to find a new integrin regulator by investigating the H-Ras-mediated integrin suppression mechanism. Through a proteomic screen using the integrin β3 cytoplasmic tail protein, we found that vimentin might work as an effector of H-Ras signaling. H-Ras converted filamentous vimentin into aggregates near the nucleus, where no integrin binding can occur. In addition, an increase in the amount of vimentin filaments accessible to the integrin β3 tail enhanced talin-induced integrin binding to its ligands by inducing integrin clustering. In contrast, the vimentin head domain, which was found to bind directly to the integrin β3 tail and compete with endogenous vimentin filaments for integrin binding, induced nuclear accumulation of vimentin filaments and reduced the amount of integrin-ligand binding. Finally, we found that expression of the vimentin head domain can reduce cell migration and metastasis. From these data, we suggest that filamentous vimentin underneath the plasma membrane is involved in increasing integrin adhesiveness, and thus regulation of the vimentin-integrin interaction might control cell adhesion.

Original languageEnglish
Pages (from-to)2030-2042
Number of pages13
JournalJournal of Cell Science
Volume129
Issue number10
DOIs
Publication statusPublished - 2016 May 15

Bibliographical note

Funding Information:
This work was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) and funded by the Ministry of Education [grant number NRF-2013R1A1A1007773]; and by a grant of the Korea Healthcare Technology R & D Project, Ministry of Health and Welfare, Republic of Korea [grant number HI14C0209].

Publisher Copyright:
© 2016.

Keywords

  • H-Ras
  • Integrin
  • Integrin activation
  • Talin
  • Vimentin

ASJC Scopus subject areas

  • Cell Biology

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