Viral antigen nanoparticles for discriminated and quantitative detection of different subtypes of anti-virus immunoglobulins

J. H. Kwon; H. H. Kim; H. B. Cho; Y. J. Cha; J. Lee

doi:10.1039/c9nr06160f

Viral antigen nanoparticles for discriminated and quantitative detection of different subtypes of anti-virus immunoglobulins

J. H. Kwon
, H. H. Kim
, H. B. Cho
, Y. J. Cha
, J. Lee^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The aim of this study is to develop a novel method for the accurate diagnosis of the infection status of viral diseases, which requires discriminated and quantitative detection of different anti-virus immunoglubulin subtypes. Considering hepatitis A as a representative model disease, viral antigen nanoparticles (vAgNPs) were designed and synthesized by genetically presenting hepatitis A virus (HAV) antigens on the surface of human heavy chain ferritin (hFTH) nanoparticles to detect anti-HAV antibodies with discriminating immunoglobulin subtypes M and G (IgM and IgG, respectively). The vAgNPs also display multi-copies of hexa-histidine peptide (H₆) on their surface to chemisorb gold ions (Au³⁺), which is vital for the autonomous generation of quantitatively meaningful detection signals. The quantitative level of anti-HAV IgM or IgG in 30 patient sera was successfully analyzed using the vAgNPs of HAV, which was performed through label-free one-step-immunoassay based on the self-enhancement of optical signals from gold nanoparticles clustered on the viral antigen nanoparticles. The diagnostic performance was compared with that of enzyme-linked immunosorbent assay (ELISA), which did not enable accurate quantitative assay due to the poor linearity between the antibody concentration and detection signal. Furthermore, these vAgNP-based immunoassays did not produce any false negative/positive signals, indicating 100% sensitivity and 100% specificity.

Original language	English
Pages (from-to)	18282-18289
Number of pages	8
Journal	Nanoscale
Volume	11
Issue number	39
DOIs	https://doi.org/10.1039/c9nr06160f
Publication status	Published - 2019 Oct 21

Bibliographical note

Funding Information:
This work was supported by the w (NRF) grant funded by the Korea government (MSIT) (No. 2019R1A2C3005771) and the Bio & Medical Technology Development Program (grant no. NRF-2017M3A9F5032628) of the National Research Foundation of Korea.

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2019R1A2C3005771) and the Bio & Medical Technology Development Program (grant no. NRF-2017M3A9F5032628) of the National Research Foundation of Korea.

Publisher Copyright:
© 2019 The Royal Society of Chemistry.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

General Materials Science

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10.1039/c9nr06160f

Cite this

@article{7817261a5d824959aa1109f3fa8bc155,

title = "Viral antigen nanoparticles for discriminated and quantitative detection of different subtypes of anti-virus immunoglobulins",

abstract = "The aim of this study is to develop a novel method for the accurate diagnosis of the infection status of viral diseases, which requires discriminated and quantitative detection of different anti-virus immunoglubulin subtypes. Considering hepatitis A as a representative model disease, viral antigen nanoparticles (vAgNPs) were designed and synthesized by genetically presenting hepatitis A virus (HAV) antigens on the surface of human heavy chain ferritin (hFTH) nanoparticles to detect anti-HAV antibodies with discriminating immunoglobulin subtypes M and G (IgM and IgG, respectively). The vAgNPs also display multi-copies of hexa-histidine peptide (H6) on their surface to chemisorb gold ions (Au3+), which is vital for the autonomous generation of quantitatively meaningful detection signals. The quantitative level of anti-HAV IgM or IgG in 30 patient sera was successfully analyzed using the vAgNPs of HAV, which was performed through label-free one-step-immunoassay based on the self-enhancement of optical signals from gold nanoparticles clustered on the viral antigen nanoparticles. The diagnostic performance was compared with that of enzyme-linked immunosorbent assay (ELISA), which did not enable accurate quantitative assay due to the poor linearity between the antibody concentration and detection signal. Furthermore, these vAgNP-based immunoassays did not produce any false negative/positive signals, indicating 100\% sensitivity and 100\% specificity.",

author = "Kwon, \{J. H.\} and Kim, \{H. H.\} and Cho, \{H. B.\} and Cha, \{Y. J.\} and J. Lee",

note = "Funding Information: This work was supported by the w (NRF) grant funded by the Korea government (MSIT) (No. 2019R1A2C3005771) and the Bio \& Medical Technology Development Program (grant no. NRF-2017M3A9F5032628) of the National Research Foundation of Korea. Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2019R1A2C3005771) and the Bio \& Medical Technology Development Program (grant no. NRF-2017M3A9F5032628) of the National Research Foundation of Korea. Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

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AU - Cho, H. B.

AU - Cha, Y. J.

AU - Lee, J.

N1 - Funding Information: This work was supported by the w (NRF) grant funded by the Korea government (MSIT) (No. 2019R1A2C3005771) and the Bio & Medical Technology Development Program (grant no. NRF-2017M3A9F5032628) of the National Research Foundation of Korea. Funding Information: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2019R1A2C3005771) and the Bio & Medical Technology Development Program (grant no. NRF-2017M3A9F5032628) of the National Research Foundation of Korea. Publisher Copyright: © 2019 The Royal Society of Chemistry.

PY - 2019/10/21

Y1 - 2019/10/21

N2 - The aim of this study is to develop a novel method for the accurate diagnosis of the infection status of viral diseases, which requires discriminated and quantitative detection of different anti-virus immunoglubulin subtypes. Considering hepatitis A as a representative model disease, viral antigen nanoparticles (vAgNPs) were designed and synthesized by genetically presenting hepatitis A virus (HAV) antigens on the surface of human heavy chain ferritin (hFTH) nanoparticles to detect anti-HAV antibodies with discriminating immunoglobulin subtypes M and G (IgM and IgG, respectively). The vAgNPs also display multi-copies of hexa-histidine peptide (H6) on their surface to chemisorb gold ions (Au3+), which is vital for the autonomous generation of quantitatively meaningful detection signals. The quantitative level of anti-HAV IgM or IgG in 30 patient sera was successfully analyzed using the vAgNPs of HAV, which was performed through label-free one-step-immunoassay based on the self-enhancement of optical signals from gold nanoparticles clustered on the viral antigen nanoparticles. The diagnostic performance was compared with that of enzyme-linked immunosorbent assay (ELISA), which did not enable accurate quantitative assay due to the poor linearity between the antibody concentration and detection signal. Furthermore, these vAgNP-based immunoassays did not produce any false negative/positive signals, indicating 100% sensitivity and 100% specificity.

AB - The aim of this study is to develop a novel method for the accurate diagnosis of the infection status of viral diseases, which requires discriminated and quantitative detection of different anti-virus immunoglubulin subtypes. Considering hepatitis A as a representative model disease, viral antigen nanoparticles (vAgNPs) were designed and synthesized by genetically presenting hepatitis A virus (HAV) antigens on the surface of human heavy chain ferritin (hFTH) nanoparticles to detect anti-HAV antibodies with discriminating immunoglobulin subtypes M and G (IgM and IgG, respectively). The vAgNPs also display multi-copies of hexa-histidine peptide (H6) on their surface to chemisorb gold ions (Au3+), which is vital for the autonomous generation of quantitatively meaningful detection signals. The quantitative level of anti-HAV IgM or IgG in 30 patient sera was successfully analyzed using the vAgNPs of HAV, which was performed through label-free one-step-immunoassay based on the self-enhancement of optical signals from gold nanoparticles clustered on the viral antigen nanoparticles. The diagnostic performance was compared with that of enzyme-linked immunosorbent assay (ELISA), which did not enable accurate quantitative assay due to the poor linearity between the antibody concentration and detection signal. Furthermore, these vAgNP-based immunoassays did not produce any false negative/positive signals, indicating 100% sensitivity and 100% specificity.

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SN - 2040-3364

VL - 11

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JO - Nanoscale

JF - Nanoscale

IS - 39

ER -

Viral antigen nanoparticles for discriminated and quantitative detection of different subtypes of anti-virus immunoglobulins

Abstract

Bibliographical note

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