@article{67742d83a64e4340b37d565ed347a83f,
title = "Viral Fitness Landscapes in Diverse Host Species Reveal Multiple Evolutionary Lines for the NS1 Gene of Influenza A Viruses",
abstract = "Mu{\~n}oz-Moreno et al. report that influenza A virus NS1 undergoes diverse and unpredictable evolutionary pathways based on its different phylogenetic lineages. A high-throughput approach using a barcoded library is used to test the interactions between NS1-recombinant viruses and to study their preference for specific or multiple hosts.",
keywords = "IFN response, NS1 evolution, barcoded library, influenza virus, innate immunity, orthomyxovirus",
author = "Raquel Mu{\~n}oz-Moreno and Carles Mart{\'i}nez-Romero and Daniel Blanco-Melo and Forst, {Christian V.} and Raffael Nachbagauer and Benitez, {Asiel Arturo} and Ignacio Mena and Sadaf Aslam and Vinod Balasubramaniam and Ilseob Lee and Maryline Panis and Juan Ayll{\'o}n and David Sachs and Park, {Man Seong} and Florian Krammer and tenOever, {Benjamin R.} and Adolfo Garc{\'i}a-Sastre",
note = "Funding Information: We thank Dr. Kimihito Ito (Hokkaido University) for sharing the R code that was used in the evolutionary direction studies. We thank Mrs. Magdalena Aichinger for her support and technical help throughout the generation and edition of the 3D landscape figures, as well as Mr. Richard Cadagan and Mr. Osman Lizardo (Icahn School of Medicine at Mount Sinai) for providing technical assistance. D.B.-M. is an Open Philanthropy Fellow of the Life Sciences Research Foundation. The figures of this work were designed to facilitate their interpretation to readers with color vision deficiencies. However, due to the complexity of specific data graphs, some panels might require color conversion software available for free, such as Visolve ( https://www.ryobi-sol.co.jp/visolve/en/ ). This research work was supported partially by CRIP (Center for Research on Influenza Pathogenesis), an NIH-NIAID-funded Center of Excellence for Influenza Research and Surveillance (CEIRS) (contract HHSN272201400008C ), and by NIH-NIAID (grants U19AI117873 and U19AI135972 ). This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF), funded by the Ministry of Science and ICT of the Republic of Korea ( NRF-2018M3A9H4056537 to M.-S.P., PI). Funding Information: We thank Dr. Kimihito Ito (Hokkaido University) for sharing the R code that was used in the evolutionary direction studies. We thank Mrs. Magdalena Aichinger for her support and technical help throughout the generation and edition of the 3D landscape figures, as well as Mr. Richard Cadagan and Mr. Osman Lizardo (Icahn School of Medicine at Mount Sinai) for providing technical assistance. D.B.-M. is an Open Philanthropy Fellow of the Life Sciences Research Foundation. The figures of this work were designed to facilitate their interpretation to readers with color vision deficiencies. However, due to the complexity of specific data graphs, some panels might require color conversion software available for free, such as Visolve (https://www.ryobi-sol.co.jp/visolve/en/). This research work was supported partially by CRIP (Center for Research on Influenza Pathogenesis), an NIH-NIAID-funded Center of Excellence for Influenza Research and Surveillance (CEIRS) (contract HHSN272201400008C), and by NIH-NIAID (grants U19AI117873 and U19AI135972). This research was also supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF), funded by the Ministry of Science and ICT of the Republic of Korea (NRF-2018M3A9H4056537 to M.-S.P. PI). Conceptualization, R.M.-M. C.M.-R. and A.G.-S.; Methodology, R.M.-M. C.M.-R. D.B.-M. C.V.F. R.N. A.A.B. I.M. S.A. V.B. I.L. M.P. J.A. and D.S.; Investigation, R.M.-M. and C.M.-R.; Writing ? Original Draft, R.M.-M. C.M.-R. and A.G.-S.; Writing ? Review & Editing, R.M.-M. C.M.-R. A.G.-S. and B.T.; Funding Acquisition, A.G.-S. and M.S.-P.; Resources, A.G.-S. M.S.-P. and F.K.; Supervision, A.G.-S. and B.R.t.O. The authors declare no competing interests. Publisher Copyright: {\textcopyright} 2019 The Author(s)",
year = "2019",
month = dec,
day = "17",
doi = "10.1016/j.celrep.2019.11.070",
language = "English",
volume = "29",
pages = "3997--4009.e5",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "12",
}