Abstract
A new polydiacetylene lipid/human olfactory receptor nano-assembly was fabricated for the visual detection of an odorant for the first time. The assembly consisted of phospholipid-mixed polydiacetylenes (PDAs) and human olfactory receptors (hORs) in detergent micelles. To overcome the limitations of bioelectronic noses, hOR-embedded chromatic complexes (PDA/hORs) were developed, introducing PDAs that showed color and fluorescence transitions against various stimuli. The chromatic nanocomplexes reacted with target molecules, showing a fluorescence intensity increase in a dose-dependent manner and target selectivity among various odorants. As a result, a color transition of the assembly from blue to purple occurred, allowing the visual detection of the odorant geraniol. Through circular dichroism (CD) spectroscopy and a tryptophan fluorescence quenching method, the structural and functional properties of the hORs embedded in the complexes were confirmed. Based on this first work, future array devices, integrating multiple nano-assemblies, can be substantiated and utilized in environmental assessment and analysis of food quality.
| Original language | English |
|---|---|
| Pages (from-to) | 7582-7587 |
| Number of pages | 6 |
| Journal | Nanoscale |
| Volume | 11 |
| Issue number | 16 |
| DOIs | |
| Publication status | Published - 2019 Apr 28 |
Bibliographical note
Funding Information:This work was supported by the National Research Foundation of Korea (NRF, 2017R1A2B3006770, 2017M3D1A1039421, and 2018R1A2B3004498) and a Korea University Grant.
Publisher Copyright:
© The Royal Society of Chemistry.
ASJC Scopus subject areas
- General Materials Science