Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly

Taegon Kim, Dongseok Moon, Jin Hyuk Park, Heehong Yang, Seongyeon Cho, Tai Hyun Park, Dong June Ahn

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    A new polydiacetylene lipid/human olfactory receptor nano-assembly was fabricated for the visual detection of an odorant for the first time. The assembly consisted of phospholipid-mixed polydiacetylenes (PDAs) and human olfactory receptors (hORs) in detergent micelles. To overcome the limitations of bioelectronic noses, hOR-embedded chromatic complexes (PDA/hORs) were developed, introducing PDAs that showed color and fluorescence transitions against various stimuli. The chromatic nanocomplexes reacted with target molecules, showing a fluorescence intensity increase in a dose-dependent manner and target selectivity among various odorants. As a result, a color transition of the assembly from blue to purple occurred, allowing the visual detection of the odorant geraniol. Through circular dichroism (CD) spectroscopy and a tryptophan fluorescence quenching method, the structural and functional properties of the hORs embedded in the complexes were confirmed. Based on this first work, future array devices, integrating multiple nano-assemblies, can be substantiated and utilized in environmental assessment and analysis of food quality.

    Original languageEnglish
    Pages (from-to)7582-7587
    Number of pages6
    JournalNanoscale
    Volume11
    Issue number16
    DOIs
    Publication statusPublished - 2019 Apr 28

    Bibliographical note

    Funding Information:
    This work was supported by the National Research Foundation of Korea (NRF, 2017R1A2B3006770, 2017M3D1A1039421, and 2018R1A2B3004498) and a Korea University Grant.

    Publisher Copyright:
    © The Royal Society of Chemistry.

    ASJC Scopus subject areas

    • General Materials Science

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