Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly

Taegon Kim; Dongseok Moon; Jin Hyuk Park; Heehong Yang; Seongyeon Cho; Tai Hyun Park; Dong June Ahn

doi:10.1039/c9nr00249a

Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly

Taegon Kim
, Dongseok Moon
, Jin Hyuk Park
, Heehong Yang
, Seongyeon Cho
, Tai Hyun Park^*
, Dong June Ahn

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A new polydiacetylene lipid/human olfactory receptor nano-assembly was fabricated for the visual detection of an odorant for the first time. The assembly consisted of phospholipid-mixed polydiacetylenes (PDAs) and human olfactory receptors (hORs) in detergent micelles. To overcome the limitations of bioelectronic noses, hOR-embedded chromatic complexes (PDA/hORs) were developed, introducing PDAs that showed color and fluorescence transitions against various stimuli. The chromatic nanocomplexes reacted with target molecules, showing a fluorescence intensity increase in a dose-dependent manner and target selectivity among various odorants. As a result, a color transition of the assembly from blue to purple occurred, allowing the visual detection of the odorant geraniol. Through circular dichroism (CD) spectroscopy and a tryptophan fluorescence quenching method, the structural and functional properties of the hORs embedded in the complexes were confirmed. Based on this first work, future array devices, integrating multiple nano-assemblies, can be substantiated and utilized in environmental assessment and analysis of food quality.

Original language	English
Pages (from-to)	7582-7587
Number of pages	6
Journal	Nanoscale
Volume	11
Issue number	16
DOIs	https://doi.org/10.1039/c9nr00249a
Publication status	Published - 2019 Apr 28

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea (NRF, 2017R1A2B3006770, 2017M3D1A1039421, and 2018R1A2B3004498) and a Korea University Grant.

Publisher Copyright:
© The Royal Society of Chemistry.

ASJC Scopus subject areas

General Materials Science

Access to Document

10.1039/c9nr00249a

Cite this

@article{baf532a522754185b4c91230b43dea27,

title = "Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly",

abstract = "A new polydiacetylene lipid/human olfactory receptor nano-assembly was fabricated for the visual detection of an odorant for the first time. The assembly consisted of phospholipid-mixed polydiacetylenes (PDAs) and human olfactory receptors (hORs) in detergent micelles. To overcome the limitations of bioelectronic noses, hOR-embedded chromatic complexes (PDA/hORs) were developed, introducing PDAs that showed color and fluorescence transitions against various stimuli. The chromatic nanocomplexes reacted with target molecules, showing a fluorescence intensity increase in a dose-dependent manner and target selectivity among various odorants. As a result, a color transition of the assembly from blue to purple occurred, allowing the visual detection of the odorant geraniol. Through circular dichroism (CD) spectroscopy and a tryptophan fluorescence quenching method, the structural and functional properties of the hORs embedded in the complexes were confirmed. Based on this first work, future array devices, integrating multiple nano-assemblies, can be substantiated and utilized in environmental assessment and analysis of food quality.",

author = "Taegon Kim and Dongseok Moon and Park, \{Jin Hyuk\} and Heehong Yang and Seongyeon Cho and Park, \{Tai Hyun\} and Ahn, \{Dong June\}",

note = "Funding Information: This work was supported by the National Research Foundation of Korea (NRF, 2017R1A2B3006770, 2017M3D1A1039421, and 2018R1A2B3004498) and a Korea University Grant. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry.",

year = "2019",

month = apr,

day = "28",

doi = "10.1039/c9nr00249a",

language = "English",

volume = "11",

pages = "7582--7587",

journal = "Nanoscale",

issn = "2040-3364",

publisher = "Royal Society of Chemistry",

number = "16",

}

TY - JOUR

T1 - Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly

AU - Kim, Taegon

AU - Moon, Dongseok

AU - Park, Jin Hyuk

AU - Yang, Heehong

AU - Cho, Seongyeon

AU - Park, Tai Hyun

AU - Ahn, Dong June

N1 - Funding Information: This work was supported by the National Research Foundation of Korea (NRF, 2017R1A2B3006770, 2017M3D1A1039421, and 2018R1A2B3004498) and a Korea University Grant. Publisher Copyright: © The Royal Society of Chemistry.

PY - 2019/4/28

Y1 - 2019/4/28

N2 - A new polydiacetylene lipid/human olfactory receptor nano-assembly was fabricated for the visual detection of an odorant for the first time. The assembly consisted of phospholipid-mixed polydiacetylenes (PDAs) and human olfactory receptors (hORs) in detergent micelles. To overcome the limitations of bioelectronic noses, hOR-embedded chromatic complexes (PDA/hORs) were developed, introducing PDAs that showed color and fluorescence transitions against various stimuli. The chromatic nanocomplexes reacted with target molecules, showing a fluorescence intensity increase in a dose-dependent manner and target selectivity among various odorants. As a result, a color transition of the assembly from blue to purple occurred, allowing the visual detection of the odorant geraniol. Through circular dichroism (CD) spectroscopy and a tryptophan fluorescence quenching method, the structural and functional properties of the hORs embedded in the complexes were confirmed. Based on this first work, future array devices, integrating multiple nano-assemblies, can be substantiated and utilized in environmental assessment and analysis of food quality.

AB - A new polydiacetylene lipid/human olfactory receptor nano-assembly was fabricated for the visual detection of an odorant for the first time. The assembly consisted of phospholipid-mixed polydiacetylenes (PDAs) and human olfactory receptors (hORs) in detergent micelles. To overcome the limitations of bioelectronic noses, hOR-embedded chromatic complexes (PDA/hORs) were developed, introducing PDAs that showed color and fluorescence transitions against various stimuli. The chromatic nanocomplexes reacted with target molecules, showing a fluorescence intensity increase in a dose-dependent manner and target selectivity among various odorants. As a result, a color transition of the assembly from blue to purple occurred, allowing the visual detection of the odorant geraniol. Through circular dichroism (CD) spectroscopy and a tryptophan fluorescence quenching method, the structural and functional properties of the hORs embedded in the complexes were confirmed. Based on this first work, future array devices, integrating multiple nano-assemblies, can be substantiated and utilized in environmental assessment and analysis of food quality.

UR - https://www.scopus.com/pages/publications/85065110059

U2 - 10.1039/c9nr00249a

DO - 10.1039/c9nr00249a

M3 - Article

C2 - 30964490

AN - SCOPUS:85065110059

SN - 2040-3364

VL - 11

SP - 7582

EP - 7587

JO - Nanoscale

JF - Nanoscale

IS - 16

ER -

Visual detection of odorant geraniol enabled by integration of a human olfactory receptor into polydiacetylene/lipid nano-assembly

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this