Visualization of RNA tertiary structure by RNA-EDTA·Fe(II) autocleavage: Analysis of tRNA(Phe) with uridine-EDTA·Fe(II) at position 47

H. Han; P. B. Dervan

doi:10.1073/pnas.91.11.4955

Visualization of RNA tertiary structure by RNA-EDTA·Fe(II) autocleavage: Analysis of tRNA(Phe) with uridine-EDTA·Fe(II) at position 47

H. Han, P. B. Dervan

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA·Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNA(Phe) in the presence of Fe(NH₄)₂(SO₄)₂ and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.

Original language	English
Pages (from-to)	4955-4959
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	91
Issue number	11
DOIs	https://doi.org/10.1073/pnas.91.11.4955
Publication status	Published - 1994
Externally published	Yes

Keywords

affinity cleaving
cleavage of RNA
yeast tRNA(Phe)

ASJC Scopus subject areas

General

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10.1073/pnas.91.11.4955

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AU - Han, H.

AU - Dervan, P. B.

PY - 1994

Y1 - 1994

N2 - To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA·Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNA(Phe) in the presence of Fe(NH4)2(SO4)2 and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.

AB - To test whether intramolecular autocleavage of RNA labeled at a single nucleotide position with EDTA·Fe(II) is a useful approach for the investigation of tertiary structures of RNAs, yeast phenylalanine tRNA was synthesized with uridine-EDTA (*U) at position U47. Autocleavage of [*U47]tRNA(Phe) in the presence of Fe(NH4)2(SO4)2 and dithiothreitol produced a set of cleavage fragments which are in general agreement with the three-dimensional structure derived from x-ray analysis.

KW - affinity cleaving

KW - cleavage of RNA

KW - yeast tRNA(Phe)

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AN - SCOPUS:0028360967

SN - 0027-8424

VL - 91

SP - 4955

EP - 4959

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 11

ER -

Visualization of RNA tertiary structure by RNA-EDTA·Fe(II) autocleavage: Analysis of tRNA(Phe) with uridine-EDTA·Fe(II) at position 47

Abstract

Keywords

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