Visualizing mitochondria and mouse intestine with a fluorescent complex of a naphthalene-based dipolar dye and serum albumin

Jong Min An, Heejo Moon, Yejin Kim, Sangrim Kang, Youngseo Kim, Yuna Jung, Sungnam Park, Peter Verwilst, B. Moon Kim, Jae Seung Kang, Dokyoung Kim

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    We have explored a new research field of fluorophores through the manipulation of fluorophore-binding proteins. The development of a new imaging agent for tracing a specific organelle or a particular site within a living organism has been of great interest in the field of basic science as well as translational medicine. In this work and for the first time, we will disclose a new naphthalene-based dipolar dye and its complex, with serum albumin (SA), and show their applicability for the selective imaging of mitochondria in cells and the intestine in a mouse. The SA-binding dipolar dye, IPNHC, was synthesized straightforwardly, and we identified its photophysical properties and binding mode with SA. IPNHC-SA complex showed a bright emission in the blue wavelength range with a high quantum yield and stability. In the fluorescence imaging study, bright fluorescence images of mouse intestines were observed under a UV light, as well as two-photon (TP) deep tissue imaging after intravenous injection of IPNHC and IPNHC-SA complex. The present findings hold great promise for the application of the fluorescent complex for use in the tracing and tracking of intestine-related diseases at clinical sites.

    Original languageEnglish
    Pages (from-to)7642-7651
    Number of pages10
    JournalJournal of Materials Chemistry B
    Volume8
    Issue number34
    DOIs
    Publication statusPublished - 2020 Sept 14

    Bibliographical note

    Publisher Copyright:
    © The Royal Society of Chemistry 2020.

    ASJC Scopus subject areas

    • General Chemistry
    • Biomedical Engineering
    • General Materials Science

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