@article{29a5fc577bc4420aa61e9e5cec289393,
title = "Visualizing mitochondria and mouse intestine with a fluorescent complex of a naphthalene-based dipolar dye and serum albumin",
abstract = "We have explored a new research field of fluorophores through the manipulation of fluorophore-binding proteins. The development of a new imaging agent for tracing a specific organelle or a particular site within a living organism has been of great interest in the field of basic science as well as translational medicine. In this work and for the first time, we will disclose a new naphthalene-based dipolar dye and its complex, with serum albumin (SA), and show their applicability for the selective imaging of mitochondria in cells and the intestine in a mouse. The SA-binding dipolar dye, IPNHC, was synthesized straightforwardly, and we identified its photophysical properties and binding mode with SA. IPNHC-SA complex showed a bright emission in the blue wavelength range with a high quantum yield and stability. In the fluorescence imaging study, bright fluorescence images of mouse intestines were observed under a UV light, as well as two-photon (TP) deep tissue imaging after intravenous injection of IPNHC and IPNHC-SA complex. The present findings hold great promise for the application of the fluorescent complex for use in the tracing and tracking of intestine-related diseases at clinical sites.",
author = "An, {Jong Min} and Heejo Moon and Yejin Kim and Sangrim Kang and Youngseo Kim and Yuna Jung and Sungnam Park and Peter Verwilst and Kim, {B. Moon} and Kang, {Jae Seung} and Dokyoung Kim",
note = "Funding Information: This research was supported by National Research Foundation (NRF) of Korea (Ministry of Science and ICT, NRF-2019-M3A9H1103783). This research was also supported by Bio & Medical Technology Development Program of the NRF of Korea (Ministry of Science & ICT, NRF-2018-M3A9H3021707) and Basic Science Research Program through the NRF of Korea (Ministry of Education, NRF-2018-R1A6A1A03025124, NRF-2018-R1D1A1B07043383). Y. K. thanks the financial support from the NRF of Korea (NRF-2017-R1A6A3A11032576). B. M. K. thanks the financial support from the NRF of Korea (NRF-2012-M3A7B4049644). P. V. acknowledges support from Interne Fondsen KU Leuven/ Internal Funds KU Leuven (STG/19/029). Funding Information: This research was supported by National Research Foundation (NRF) of Korea (Ministry of Science and ICT, NRF-2019-M3A9H1103783). This research was also supported by Bio & Medical Technology Development Program of the NRF of Korea (Ministry of Science & ICT, NRF-2018-M3A9H3021707) and Basic Science Research Program through the NRF of Korea (Ministry of Education, NRF-2018-R1A6A1A03025124, NRF-2018-R1D1A1B07043383). Y. K. thanks the financial support from the NRF of Korea (NRF-2017-R1A6A3A11032576). B. M. K. thanks the financial support from the NRF of Korea (NRF-2012-M3A7B4049644). P. V. acknowledges support from Interne Fondsen KU Leuven/Internal Funds KU Leuven (STG/19/029). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2020.",
year = "2020",
month = sep,
day = "14",
doi = "10.1039/d0tb01314e",
language = "English",
volume = "8",
pages = "7642--7651",
journal = "Journal of Materials Chemistry B",
issn = "2050-750X",
publisher = "Royal Society of Chemistry",
number = "34",
}