Abstract
Two conjugated polyelectrolytes with same electronic structure with/without molecular spacer (or bumper) are compared in functioning as a donor in fluorescence resonance energy transfer (FRET) to a fluorescein-labeled single-stranded DNA (ssDNA-Fl). Fluorescence quenching of the acceptor (Fl) in the polymer/ssDNA-Fl electrostatic complex is a main concern to improve detection sensitivity. This study can show one important example of structure-property relationships for the FRET-based biosensing assays. Structure modification such as molecular bumper can fine-tune the competition between energy transfer and electron transfer quenching in FRET-based DNA sequence detection.
Original language | English |
---|---|
Pages (from-to) | 244/[1286]-249/[1291] |
Journal | Molecular Crystals and Liquid Crystals |
Volume | 486 |
DOIs | |
Publication status | Published - 2008 |
Externally published | Yes |
Bibliographical note
Funding Information:This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2006-331-D00139) and Pusan National University Research Grant, 2006.
Keywords
- Biosensor
- Conjugated polyelectrolyte
- DNA detection
- DNA sensor
- FRET
ASJC Scopus subject areas
- General Chemistry
- General Materials Science
- Condensed Matter Physics