Water-soluble polyelectrolytes for FRET-based DNA detection

Han Young Woo; Okhil Kumar Nag; Jinseck Kim; Mijeong Kang; Guillermo C. Bazan

doi:10.1080/15421400801921694

Water-soluble polyelectrolytes for FRET-based DNA detection

Han Young Woo, Okhil Kumar Nag, Jinseck Kim, Mijeong Kang, Guillermo C. Bazan

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Two conjugated polyelectrolytes with same electronic structure with/without molecular spacer (or bumper) are compared in functioning as a donor in fluorescence resonance energy transfer (FRET) to a fluorescein-labeled single-stranded DNA (ssDNA-Fl). Fluorescence quenching of the acceptor (Fl) in the polymer/ssDNA-Fl electrostatic complex is a main concern to improve detection sensitivity. This study can show one important example of structure-property relationships for the FRET-based biosensing assays. Structure modification such as molecular bumper can fine-tune the competition between energy transfer and electron transfer quenching in FRET-based DNA sequence detection.

Original language	English
Pages (from-to)	244/[1286]-249/[1291]
Journal	Molecular Crystals and Liquid Crystals
Volume	486
DOIs	https://doi.org/10.1080/15421400801921694
Publication status	Published - 2008
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2006-331-D00139) and Pusan National University Research Grant, 2006.

Keywords

Biosensor
Conjugated polyelectrolyte
DNA detection
DNA sensor
FRET

ASJC Scopus subject areas

General Chemistry
General Materials Science
Condensed Matter Physics

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10.1080/15421400801921694

Cite this

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title = "Water-soluble polyelectrolytes for FRET-based DNA detection",

abstract = "Two conjugated polyelectrolytes with same electronic structure with/without molecular spacer (or bumper) are compared in functioning as a donor in fluorescence resonance energy transfer (FRET) to a fluorescein-labeled single-stranded DNA (ssDNA-Fl). Fluorescence quenching of the acceptor (Fl) in the polymer/ssDNA-Fl electrostatic complex is a main concern to improve detection sensitivity. This study can show one important example of structure-property relationships for the FRET-based biosensing assays. Structure modification such as molecular bumper can fine-tune the competition between energy transfer and electron transfer quenching in FRET-based DNA sequence detection.",

keywords = "Biosensor, Conjugated polyelectrolyte, DNA detection, DNA sensor, FRET",

author = "Woo, {Han Young} and Nag, {Okhil Kumar} and Jinseck Kim and Mijeong Kang and Bazan, {Guillermo C.}",

note = "Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2006-331-D00139) and Pusan National University Research Grant, 2006.",

year = "2008",

doi = "10.1080/15421400801921694",

language = "English",

volume = "486",

pages = "244/[1286]--249/[1291]",

journal = "Molecular Crystals and Liquid Crystals",

issn = "1542-1406",

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T1 - Water-soluble polyelectrolytes for FRET-based DNA detection

AU - Woo, Han Young

AU - Nag, Okhil Kumar

AU - Kim, Jinseck

AU - Kang, Mijeong

AU - Bazan, Guillermo C.

N1 - Funding Information: This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund) (KRF-2006-331-D00139) and Pusan National University Research Grant, 2006.

PY - 2008

Y1 - 2008

N2 - Two conjugated polyelectrolytes with same electronic structure with/without molecular spacer (or bumper) are compared in functioning as a donor in fluorescence resonance energy transfer (FRET) to a fluorescein-labeled single-stranded DNA (ssDNA-Fl). Fluorescence quenching of the acceptor (Fl) in the polymer/ssDNA-Fl electrostatic complex is a main concern to improve detection sensitivity. This study can show one important example of structure-property relationships for the FRET-based biosensing assays. Structure modification such as molecular bumper can fine-tune the competition between energy transfer and electron transfer quenching in FRET-based DNA sequence detection.

AB - Two conjugated polyelectrolytes with same electronic structure with/without molecular spacer (or bumper) are compared in functioning as a donor in fluorescence resonance energy transfer (FRET) to a fluorescein-labeled single-stranded DNA (ssDNA-Fl). Fluorescence quenching of the acceptor (Fl) in the polymer/ssDNA-Fl electrostatic complex is a main concern to improve detection sensitivity. This study can show one important example of structure-property relationships for the FRET-based biosensing assays. Structure modification such as molecular bumper can fine-tune the competition between energy transfer and electron transfer quenching in FRET-based DNA sequence detection.

KW - Biosensor

KW - Conjugated polyelectrolyte

KW - DNA detection

KW - DNA sensor

KW - FRET

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JF - Molecular Crystals and Liquid Crystals

ER -

Water-soluble polyelectrolytes for FRET-based DNA detection

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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