WDNM1 is associated with differentiation and apoptosis of mammary epithelial cells

Yoonjung Kho; Sungchan Kim; Byung Sun Yoon; Jai Hee Moon; Sungwook Kwak; Gyuman Park; Junghee Woo; Sejong Oh; Kichang Hong; Saehun Kim; Hyunggee Kim; Seungkwon You; Yunjaie Choi

doi:10.1080/10495390801887361

WDNM1 is associated with differentiation and apoptosis of mammary epithelial cells

Yoonjung Kho, Sungchan Kim, Byung Sun Yoon, Jai Hee Moon, Sungwook Kwak, Gyuman Park, Junghee Woo, Sejong Oh, Kichang Hong, Saehun Kim, Hyunggee Kim, Seungkwon You, Yunjaie Choi

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNF treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNF induced WDNM1 expression showed the NFB-dependent mechanism since it's expression was abrogated in IBM (super-repressor of NFB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNF-NFB signal pathway, is associated with HC11 cell apoptosis.

Original language	English
Pages (from-to)	89-103
Number of pages	15
Journal	Animal Biotechnology
Volume	19
Issue number	2
DOIs	https://doi.org/10.1080/10495390801887361
Publication status	Published - 2008 Apr

Keywords

Apoptosis
Mammary epithelial cell
NFκB
TNFα
WDNM1

ASJC Scopus subject areas

Biotechnology
Bioengineering
Animal Science and Zoology

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10.1080/10495390801887361

Cite this

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title = "WDNM1 is associated with differentiation and apoptosis of mammary epithelial cells",

abstract = "In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNF treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNF induced WDNM1 expression showed the NFB-dependent mechanism since it's expression was abrogated in IBM (super-repressor of NFB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNF-NFB signal pathway, is associated with HC11 cell apoptosis.",

keywords = "Apoptosis, Mammary epithelial cell, NFκB, TNFα, WDNM1",

author = "Yoonjung Kho and Sungchan Kim and Yoon, {Byung Sun} and Moon, {Jai Hee} and Sungwook Kwak and Gyuman Park and Junghee Woo and Sejong Oh and Kichang Hong and Saehun Kim and Hyunggee Kim and Seungkwon You and Yunjaie Choi",

year = "2008",

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doi = "10.1080/10495390801887361",

language = "English",

volume = "19",

pages = "89--103",

journal = "Animal Biotechnology",

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T1 - WDNM1 is associated with differentiation and apoptosis of mammary epithelial cells

AU - Kho, Yoonjung

AU - Kim, Sungchan

AU - Yoon, Byung Sun

AU - Moon, Jai Hee

AU - Kwak, Sungwook

AU - Park, Gyuman

AU - Woo, Junghee

AU - Oh, Sejong

AU - Hong, Kichang

AU - Kim, Saehun

AU - Kim, Hyunggee

AU - You, Seungkwon

AU - Choi, Yunjaie

PY - 2008/4

Y1 - 2008/4

N2 - In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNF treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNF induced WDNM1 expression showed the NFB-dependent mechanism since it's expression was abrogated in IBM (super-repressor of NFB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNF-NFB signal pathway, is associated with HC11 cell apoptosis.

AB - In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNF treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNF induced WDNM1 expression showed the NFB-dependent mechanism since it's expression was abrogated in IBM (super-repressor of NFB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNF-NFB signal pathway, is associated with HC11 cell apoptosis.

KW - Apoptosis

KW - Mammary epithelial cell

KW - NFκB

KW - TNFα

KW - WDNM1

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U2 - 10.1080/10495390801887361

DO - 10.1080/10495390801887361

M3 - Article

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AN - SCOPUS:42649088130

SN - 1049-5398

VL - 19

SP - 89

EP - 103

JO - Animal Biotechnology

JF - Animal Biotechnology

IS - 2

ER -

WDNM1 is associated with differentiation and apoptosis of mammary epithelial cells

Abstract

Keywords

ASJC Scopus subject areas

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