In this study, we show that expression of the Westmead DMBA8 nonmetastatic cDNA 1 (WDNM1) gene was increased upon SFM and/or TNF treatment, with a corresponding increase in apoptotic cells, and gradually decreased following re-stimulation with serum in HC11 mammary epithelial cells. TNF induced WDNM1 expression showed the NFB-dependent mechanism since it's expression was abrogated in IBM (super-repressor of NFB)-transfected cells, but not those transfected with control vector. Furthermore, overexpression of WDNM1 suppressed growth and differentiation, and accelerated apoptosis of HC11 cells. Thus, our results demonstrate that WDNM1 gene expression, regulated by the TNF-NFB signal pathway, is associated with HC11 cell apoptosis.
|Number of pages||15|
|Publication status||Published - 2008 Apr|
- Mammary epithelial cell
ASJC Scopus subject areas
- Animal Science and Zoology