Wheat (Triticum aestivum. L) Plant U-box E3 ligases TaPUB2 and TaPUB3 enhance ABA response and salt stress resistance in Arabidopsis

Moon Seok Kim, Jae Ho Kim, Joseph Noble Amoah, Yong Weon Seo

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Plant U-box E3 ligases (PUBs) are important regulators of responses to various abiotic stress conditions. In this study, we found that wheat (Triticum aestivum. L) PUBs TaPUB2 and TaPUB3 enhanced abscisic acid (ABA) responses and salt tolerance in Arabidopsis. We generated transgenic Arabidopsis lines overexpressing TaPUB2 and TaPUB3 and performed various plant physiological experiments. Overexpression of TaPUB2 and TaPUB3 increased tolerance to salinity stress in an ABA-dependent manner in transgenic plants, as evidenced by germination and survival rates, root length, stomatal aperture regulation, membrane peroxidation, photosynthetic activities, reactive oxygen species scavenging activities and expression of various ABA and salinity stress-related genes. These results demonstrate the functions of PUBs under ABA and salinity stress conditions and provide valuable information for the development of salinity stress-tolerant crop species.

Original languageEnglish
Pages (from-to)3037-3050
Number of pages14
JournalFEBS Letters
Volume596
Issue number23
DOIs
Publication statusPublished - 2022 Dec

Bibliographical note

Funding Information:
This study was supported by a grant from the Korea University and Basic Science Research Program through the National Research Foundation of Korea, funded by the Ministry of Science, ICT & Future Planning (grant number 2017R1D1A1B06030349). The authors would like to thank Prof. Jong Yun Kim for providing CIRAS‐3.

Publisher Copyright:
© 2022 Federation of European Biochemical Societies.

Keywords

  • Plant U-box protein
  • abscisic acid
  • salt stress

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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