Wheat (Triticum aestivum l.) tahmw1d transcript variants are highly expressed in response to heat stress and in grains located in distal part of the spike

Chan Seop Ko, Jin Baek Kim, Min Jeong Hong, Yong Weon Seo

    Research output: Contribution to journalArticlepeer-review

    1 Citation (Scopus)

    Abstract

    High-temperature stress during the grain filling stage has a deleterious effect on grain yield and end-use quality. Plants undergo various transcriptional events of protein complexity as defensive responses to various stressors. The “Keumgang” wheat cultivar was subjected to high-temperature stress for 6 and 10 days beginning 9 days after anthesis, then two-dimensional gel electrophoresis (2DE) and peptide analyses were performed. Spots showing decreased contents in stressed plants were shown to have strong similarities with a high-molecular glutenin gene, TraesCS1D02G317301 (TaHMW1D). QRT-PCR results confirmed that TaHMW1D was expressed in its full form and in the form of four different transcript variants. These events always occurred between repetitive regions at specific deletion sites (5-CAA (Glutamine) GG/TG (Glycine) or (Valine)-3, 5-GGG (Glycine) CAA (Glutamine)-3 ) in an exonic region. Heat stress led to a significant increase in the expression of the transcript variants. This was most evident in the distal parts of the spike. Considering the importance of high-molecular weight glutenin subunits of seed storage proteins, stressed plants might choose shorter polypeptides while retaining glutenin function, thus maintaining the expression of glutenin motifs and conserved sites.

    Original languageEnglish
    Article number687
    JournalPlants
    Volume10
    Issue number4
    DOIs
    Publication statusPublished - 2021 Apr

    Bibliographical note

    Funding Information:
    Funding: This research was funded by a Cooperative Research Program for Agriculture Science & Technology Development (PJ015666) funded by Rural Development Administration, Republic of Korea. This research was also supported by a grant from the Korea University.

    Publisher Copyright:
    © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

    Keywords

    • Grain-filling period
    • High temperature stress
    • High-molecular glutenin gene
    • Spikelet position in spike
    • Transcript variants
    • Wheat

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Ecology
    • Plant Science

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