Wheat truncated hemoglobin interacts with photosystem I PSK-I subunit and photosystem II subunit PsbS1

D. Y. Kim; M. J. Hong; Y. J. Lee; M. B. Lee; Y. W. Seo

doi:10.1007/s10535-012-0268-y

Wheat truncated hemoglobin interacts with photosystem I PSK-I subunit and photosystem II subunit PsbS1

D. Y. Kim
, M. J. Hong
, Y. J. Lee
, M. B. Lee
, Y. W. Seo

Department of Biosystems and Biotechnology

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Recently, the truncated hemoglobin gene (trHb) was discovered in plant species, however, its role has not yet been determined. In this study, the gene expression of wheat trHb (TatrHb) was analyzed under various biotic and abiotic stresses. TatrHb transcript levels increased in NaCl-treated leaves and gibberellic acid (GA₃)-treated roots. In addition, sodium nitroprusside (SNP), a nitric oxide donor, induced an increase in TatrHb transcript levels in roots and leaves. A yeast two-hybrid assay (YIIH) was used to screen a hypoxia-treated wheat seedling library with the goal of determining the putative function of TatrHb. In this YIIH assay, photosynthesis-related genes that showed high homology to the Hordeum vulgare chloroplast photosystem I PSK-I subunit and Zea mays photosystem II subunit PsbS1 were detected and their interactions with TatrHb were confirmed. Subcellular localization of a TatrHb-green fluorescent protein (GFP) fusion protein and bimolecular fluorescence complementation (BiFC) assay suggested that TatrHb is involved in photosynthesis. The TatrHb-GFP fusion protein was localized in the plastids and the yellow fluorescent protein signal indicated that the TatrHb protein interacted with PSK-I and PsbS1 in the chloroplast.

Original language	English
Pages (from-to)	281-290
Number of pages	10
Journal	Biologia Plantarum
Volume	57
Issue number	2
DOIs	https://doi.org/10.1007/s10535-012-0268-y
Publication status	Published - 2013 Jun 1

Bibliographical note

Funding Information:
Received 3 January 2012, accepted 12 June 2012. Abbreviations: ABA - abscisic acid; BiFC - bimolecular fluorescence complementation; c-PTIO - 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3 oxidase; GA3 - gibberellic acid; GFP - green fluorescent protein; Hb - hemoglobin; MeJA - methyl jasmonate; NOS - NO synthase; ONPG - O-nitrophenyl β-D-galactopyranoside; PEG - polyethylene glycol; RT-PCR - reverse transcriptase-polymerase chain reaction; SA - salicylic acid; SNP - sodium nitroprusside; TatrHb - Triticum aestivum L. truncated hemoglobin; YIIH - yeast 2-hybrid; YFP - yellow fluorescent protein. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ008031), Rural Development Administration, Republic of Korea. * Corresponding author; fax: (+82) 2 3290 3501, e-mail: [email protected]

Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.

Keywords

NaCl
Triticum aestivum
YIIH assay
chloroplast
gibberellic acid
nitric oxide
photosynthesis

ASJC Scopus subject areas

Plant Science
Horticulture

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10.1007/s10535-012-0268-y

Cite this

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title = "Wheat truncated hemoglobin interacts with photosystem I PSK-I subunit and photosystem II subunit PsbS1",

abstract = "Recently, the truncated hemoglobin gene (trHb) was discovered in plant species, however, its role has not yet been determined. In this study, the gene expression of wheat trHb (TatrHb) was analyzed under various biotic and abiotic stresses. TatrHb transcript levels increased in NaCl-treated leaves and gibberellic acid (GA3)-treated roots. In addition, sodium nitroprusside (SNP), a nitric oxide donor, induced an increase in TatrHb transcript levels in roots and leaves. A yeast two-hybrid assay (YIIH) was used to screen a hypoxia-treated wheat seedling library with the goal of determining the putative function of TatrHb. In this YIIH assay, photosynthesis-related genes that showed high homology to the Hordeum vulgare chloroplast photosystem I PSK-I subunit and Zea mays photosystem II subunit PsbS1 were detected and their interactions with TatrHb were confirmed. Subcellular localization of a TatrHb-green fluorescent protein (GFP) fusion protein and bimolecular fluorescence complementation (BiFC) assay suggested that TatrHb is involved in photosynthesis. The TatrHb-GFP fusion protein was localized in the plastids and the yellow fluorescent protein signal indicated that the TatrHb protein interacted with PSK-I and PsbS1 in the chloroplast.",

keywords = "NaCl, Triticum aestivum, YIIH assay, chloroplast, gibberellic acid, nitric oxide, photosynthesis",

author = "Kim, \{D. Y.\} and Hong, \{M. J.\} and Lee, \{Y. J.\} and Lee, \{M. B.\} and Seo, \{Y. W.\}",

note = "Funding Information: Received 3 January 2012, accepted 12 June 2012. Abbreviations: ABA - abscisic acid; BiFC - bimolecular fluorescence complementation; c-PTIO - 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3 oxidase; GA3 - gibberellic acid; GFP - green fluorescent protein; Hb - hemoglobin; MeJA - methyl jasmonate; NOS - NO synthase; ONPG - O-nitrophenyl β-D-galactopyranoside; PEG - polyethylene glycol; RT-PCR - reverse transcriptase-polymerase chain reaction; SA - salicylic acid; SNP - sodium nitroprusside; TatrHb - Triticum aestivum L. truncated hemoglobin; YIIH - yeast 2-hybrid; YFP - yellow fluorescent protein. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ008031), Rural Development Administration, Republic of Korea. * Corresponding author; fax: (+82) 2 3290 3501, e-mail: [email protected] Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2013",

month = jun,

day = "1",

doi = "10.1007/s10535-012-0268-y",

language = "English",

volume = "57",

pages = "281--290",

journal = "Biologia Plantarum",

issn = "0006-3134",

publisher = "Springer Netherlands",

number = "2",

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TY - JOUR

T1 - Wheat truncated hemoglobin interacts with photosystem I PSK-I subunit and photosystem II subunit PsbS1

AU - Kim, D. Y.

AU - Hong, M. J.

AU - Lee, Y. J.

AU - Lee, M. B.

AU - Seo, Y. W.

N1 - Funding Information: Received 3 January 2012, accepted 12 June 2012. Abbreviations: ABA - abscisic acid; BiFC - bimolecular fluorescence complementation; c-PTIO - 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3 oxidase; GA3 - gibberellic acid; GFP - green fluorescent protein; Hb - hemoglobin; MeJA - methyl jasmonate; NOS - NO synthase; ONPG - O-nitrophenyl β-D-galactopyranoside; PEG - polyethylene glycol; RT-PCR - reverse transcriptase-polymerase chain reaction; SA - salicylic acid; SNP - sodium nitroprusside; TatrHb - Triticum aestivum L. truncated hemoglobin; YIIH - yeast 2-hybrid; YFP - yellow fluorescent protein. Acknowledgement: This work was supported by a grant from the Next-Generation BioGreen 21 Program (Plant Molecular Breeding Center No. PJ008031), Rural Development Administration, Republic of Korea. * Corresponding author; fax: (+82) 2 3290 3501, e-mail: [email protected] Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2013/6/1

Y1 - 2013/6/1

N2 - Recently, the truncated hemoglobin gene (trHb) was discovered in plant species, however, its role has not yet been determined. In this study, the gene expression of wheat trHb (TatrHb) was analyzed under various biotic and abiotic stresses. TatrHb transcript levels increased in NaCl-treated leaves and gibberellic acid (GA3)-treated roots. In addition, sodium nitroprusside (SNP), a nitric oxide donor, induced an increase in TatrHb transcript levels in roots and leaves. A yeast two-hybrid assay (YIIH) was used to screen a hypoxia-treated wheat seedling library with the goal of determining the putative function of TatrHb. In this YIIH assay, photosynthesis-related genes that showed high homology to the Hordeum vulgare chloroplast photosystem I PSK-I subunit and Zea mays photosystem II subunit PsbS1 were detected and their interactions with TatrHb were confirmed. Subcellular localization of a TatrHb-green fluorescent protein (GFP) fusion protein and bimolecular fluorescence complementation (BiFC) assay suggested that TatrHb is involved in photosynthesis. The TatrHb-GFP fusion protein was localized in the plastids and the yellow fluorescent protein signal indicated that the TatrHb protein interacted with PSK-I and PsbS1 in the chloroplast.

AB - Recently, the truncated hemoglobin gene (trHb) was discovered in plant species, however, its role has not yet been determined. In this study, the gene expression of wheat trHb (TatrHb) was analyzed under various biotic and abiotic stresses. TatrHb transcript levels increased in NaCl-treated leaves and gibberellic acid (GA3)-treated roots. In addition, sodium nitroprusside (SNP), a nitric oxide donor, induced an increase in TatrHb transcript levels in roots and leaves. A yeast two-hybrid assay (YIIH) was used to screen a hypoxia-treated wheat seedling library with the goal of determining the putative function of TatrHb. In this YIIH assay, photosynthesis-related genes that showed high homology to the Hordeum vulgare chloroplast photosystem I PSK-I subunit and Zea mays photosystem II subunit PsbS1 were detected and their interactions with TatrHb were confirmed. Subcellular localization of a TatrHb-green fluorescent protein (GFP) fusion protein and bimolecular fluorescence complementation (BiFC) assay suggested that TatrHb is involved in photosynthesis. The TatrHb-GFP fusion protein was localized in the plastids and the yellow fluorescent protein signal indicated that the TatrHb protein interacted with PSK-I and PsbS1 in the chloroplast.

KW - NaCl

KW - Triticum aestivum

KW - YIIH assay

KW - chloroplast

KW - gibberellic acid

KW - nitric oxide

KW - photosynthesis

UR - https://www.scopus.com/pages/publications/84876080136

U2 - 10.1007/s10535-012-0268-y

DO - 10.1007/s10535-012-0268-y

M3 - Article

AN - SCOPUS:84876080136

SN - 0006-3134

VL - 57

SP - 281

EP - 290

JO - Biologia Plantarum

JF - Biologia Plantarum

IS - 2

ER -

Wheat truncated hemoglobin interacts with photosystem I PSK-I subunit and photosystem II subunit PsbS1

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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