Wide-field reflection phase microscope

Zahid Yaqoob, Toyohiko Yamauchi, Dan Fu, Wonshik Choi, Ramachandra R. Dasari, Michael S. Feld

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Wide-field reflection phase microscopy is highly desired for depth-resolved measurement of cellular structures without the need for raster scanning. We report a low coherence reflection phase microscope based on time-domain optical coherence tomography and off-axis interferometry. The setup uniquely provides the desired angular shift to the reference beam for off-axis interferometry while promising equal path length across the whole reference beam. We show subnanometer path-length sensitivity of our instrument and demonstrate high-speed imaging of membrane fluctuations in eukaryotic cells.

    Original languageEnglish
    Title of host publicationThree-Dimensional and Multidimensional Microscopy
    Subtitle of host publicationImage Acquisition and Processing XVIII
    DOIs
    Publication statusPublished - 2011
    EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII - San Francisco, CA, United States
    Duration: 2011 Jan 242011 Jan 27

    Publication series

    NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
    Volume7904
    ISSN (Print)1605-7422

    Other

    OtherThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVIII
    Country/TerritoryUnited States
    CitySan Francisco, CA
    Period11/1/2411/1/27

    Keywords

    • Low coherence interferometry
    • Optical coherence tomography
    • Quantitative phase imaging

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Biomaterials
    • Atomic and Molecular Physics, and Optics
    • Radiology Nuclear Medicine and imaging

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