Wnt ligand binding to and regulation of dopamine D2 receptors

Sehyoun Yoon, Mi Hyun Choi, Ja Hyun Baik

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The dopamine D2 receptor (D2R) plays an important role in mesencephalic dopaminergic neuronal development, and we have previously reported that Wnt5a interacts with D2R to promote the differentiation of dopaminergic neurons. Co-immunoprecipitation of Wnt5a and D2R, displacement of the D2R receptor antagonist [3H]spiperone from D2R by Wnt5a, as well as glutathione S-transferase (GST) pulldown assays demonstrated that Wnt5a binds D2R. In this chapter, we present the co-immunoprecipitation, pull- down, and competition-binding assays used in the analysis of the D2R–Wnt5a interaction.

Original languageEnglish
Pages (from-to)65-78
Number of pages14
JournalNeuromethods
Volume96
DOIs
Publication statusPublished - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

  • Co-immunoprecipitation
  • Dopamine D2 receptor
  • GST pull-down assay
  • Radioligand binding
  • Wnt5a

ASJC Scopus subject areas

  • General Neuroscience
  • General Biochemistry,Genetics and Molecular Biology
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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