Xk-related protein 8 regulates myoblast differentiation and survival

Go Woon Kim, Gi Hoon Nam, In San Kim, Seung Yoon Park

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


Xk-related protein 8 (Xkr8) is a scramblase and responsible for phosphatidylserine (PS) exposure on the cell surface in a caspase-dependent manner. Although PS exposure is found to be important for myotube formation during myoblast differentiation, the role of Xkr8 during myogenesis has not been elucidated. Here we show that Xkr8 contributes to myoblast differentiation. Xkr8 overexpression induced the formation of large myotubes during early differentiation, but this phenotype was not related to caspase-dependent cleavage of Xkr8. Furthermore, forced Xkr8 expression accelerated myoblast differentiation and conferred cell-death resistance after the induction of differentiation. Consistent with these results, Xkr8-knocked-down myoblasts exhibited impaired differentiation and more apoptotic cells during differentiation, implying the involvements of Xkr8 in the survival and proliferation of myoblasts. Taken together, the study shows Xkr8 influences myogenesis by acting as a positive regulator of terminal differentiation and myoblast survival.

Original languageEnglish
Pages (from-to)3575-3588
Number of pages14
JournalFEBS Journal
Issue number21
Publication statusPublished - 2017 Nov


  • Xk-related protein 8
  • apoptosis
  • differentiation
  • myoblasts
  • scramblase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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