Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA

Ki Moon Seong; Sang Oun Jung; Hag Dong Kim; Hee Ju Kim; You Jin Jung; Soo Young Choi; Joon Kim

doi:10.1016/j.febslet.2011.12.030

Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA

Ki Moon Seong, Sang Oun Jung, Hag Dong Kim, Hee Ju Kim, You Jin Jung, Soo Young Choi, Joon Kim

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Yeast ribosomal protein S3 has multifunctional activities that are involved in both protein translation and DNA repair. Here, we report that yeast Rps3p cleaves variously damaged DNA that contains not only AP sites and pyrimidine dimers but also 7,8-hydro-8-oxoguanine. This study also revealed that Rps3p has a β-lyase activity with a broad range of substrate specificity which cleaves phosphodiester bonds of UV or oxidatively damaged DNA substrates. Mutation analysis of the yeast Rps3 protein including introduction of domain deletions and residue replacements identified the residues Asp154 and Lys200 are important for the catalytic activity. In addition, the repair enzyme activity of yeast Rps3p was confirmed by complementation in xth, nfo Escherichia coli cells in which the DNA repair process is defective.

Original language	English
Pages (from-to)	356-361
Number of pages	6
Journal	FEBS Letters
Volume	586
Issue number	4
DOIs	https://doi.org/10.1016/j.febslet.2011.12.030
Publication status	Published - 2012 Feb 17

Keywords

8-Oxo-G
AP sites
DNA repair
Lyase
Pyrimidine dimer
Yeast Rps3p

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2011.12.030

Cite this

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title = "Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA",

abstract = "Yeast ribosomal protein S3 has multifunctional activities that are involved in both protein translation and DNA repair. Here, we report that yeast Rps3p cleaves variously damaged DNA that contains not only AP sites and pyrimidine dimers but also 7,8-hydro-8-oxoguanine. This study also revealed that Rps3p has a β-lyase activity with a broad range of substrate specificity which cleaves phosphodiester bonds of UV or oxidatively damaged DNA substrates. Mutation analysis of the yeast Rps3 protein including introduction of domain deletions and residue replacements identified the residues Asp154 and Lys200 are important for the catalytic activity. In addition, the repair enzyme activity of yeast Rps3p was confirmed by complementation in xth, nfo Escherichia coli cells in which the DNA repair process is defective.",

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author = "Seong, {Ki Moon} and Jung, {Sang Oun} and Kim, {Hag Dong} and Kim, {Hee Ju} and Jung, {You Jin} and Choi, {Soo Young} and Joon Kim",

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AU - Seong, Ki Moon

AU - Jung, Sang Oun

AU - Kim, Hag Dong

AU - Kim, Hee Ju

AU - Jung, You Jin

AU - Choi, Soo Young

AU - Kim, Joon

PY - 2012/2/17

Y1 - 2012/2/17

N2 - Yeast ribosomal protein S3 has multifunctional activities that are involved in both protein translation and DNA repair. Here, we report that yeast Rps3p cleaves variously damaged DNA that contains not only AP sites and pyrimidine dimers but also 7,8-hydro-8-oxoguanine. This study also revealed that Rps3p has a β-lyase activity with a broad range of substrate specificity which cleaves phosphodiester bonds of UV or oxidatively damaged DNA substrates. Mutation analysis of the yeast Rps3 protein including introduction of domain deletions and residue replacements identified the residues Asp154 and Lys200 are important for the catalytic activity. In addition, the repair enzyme activity of yeast Rps3p was confirmed by complementation in xth, nfo Escherichia coli cells in which the DNA repair process is defective.

AB - Yeast ribosomal protein S3 has multifunctional activities that are involved in both protein translation and DNA repair. Here, we report that yeast Rps3p cleaves variously damaged DNA that contains not only AP sites and pyrimidine dimers but also 7,8-hydro-8-oxoguanine. This study also revealed that Rps3p has a β-lyase activity with a broad range of substrate specificity which cleaves phosphodiester bonds of UV or oxidatively damaged DNA substrates. Mutation analysis of the yeast Rps3 protein including introduction of domain deletions and residue replacements identified the residues Asp154 and Lys200 are important for the catalytic activity. In addition, the repair enzyme activity of yeast Rps3p was confirmed by complementation in xth, nfo Escherichia coli cells in which the DNA repair process is defective.

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KW - AP sites

KW - DNA repair

KW - Lyase

KW - Pyrimidine dimer

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Yeast ribosomal protein S3 possesses a β-lyase activity on damaged DNA

Abstract

Keywords

ASJC Scopus subject areas

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