Zinc insulin hexamer loaded alginate zinc hydrogel: Preparation, characterization and in vivo hypoglycemic ability

Haoran Cheng; Haishan Wu; Ting Guo; Hyun Jin Park; Jinglei Li

doi:10.1016/j.ejpb.2022.08.016

Zinc insulin hexamer loaded alginate zinc hydrogel: Preparation, characterization and in vivo hypoglycemic ability

Haoran Cheng, Haishan Wu, Ting Guo, Hyun Jin Park, Jinglei Li

Department of Biotechnology

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Alginate zinc hydrogel loaded with zinc insulin hexamer was prepared and characterized for oral insulin administration. The hydrogel was fabricated by dripping zinc insulin hexamer into sodium alginate solution and followed by crosslinking by zinc chloride. SEM image reveals the zinc insulin hexamer was integrated into the matrix of hydrogel. Zinc insulin hexamer loaded hydrogel shows no obvious cytotoxicity to both HT29 and Caco-2 cells. The developed hydrogel retards the burst release of insulin in simulated gastric fluid but promotes the release when in simulated intestinal fluid. In the diabetic mice, zinc insulin hexamer loaded alginate hydrogel demonstrates significant and prolonged hypoglycemic effect.

Original language	English
Pages (from-to)	173-181
Number of pages	9
Journal	European Journal of Pharmaceutics and Biopharmaceutics
Volume	179
DOIs	https://doi.org/10.1016/j.ejpb.2022.08.016
Publication status	Published - 2022 Oct

Bibliographical note

Funding Information:
This work is supported by National Natural Science Foundation of China (31700015).

Publisher Copyright:
© 2022 Elsevier B.V.

Keywords

Control release
Hydrogel
Insulin
Maltodextrin
Sodium alginate

ASJC Scopus subject areas

Biotechnology
Pharmaceutical Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ejpb.2022.08.016

Cite this

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title = "Zinc insulin hexamer loaded alginate zinc hydrogel: Preparation, characterization and in vivo hypoglycemic ability",

abstract = "Alginate zinc hydrogel loaded with zinc insulin hexamer was prepared and characterized for oral insulin administration. The hydrogel was fabricated by dripping zinc insulin hexamer into sodium alginate solution and followed by crosslinking by zinc chloride. SEM image reveals the zinc insulin hexamer was integrated into the matrix of hydrogel. Zinc insulin hexamer loaded hydrogel shows no obvious cytotoxicity to both HT29 and Caco-2 cells. The developed hydrogel retards the burst release of insulin in simulated gastric fluid but promotes the release when in simulated intestinal fluid. In the diabetic mice, zinc insulin hexamer loaded alginate hydrogel demonstrates significant and prolonged hypoglycemic effect.",

keywords = "Control release, Hydrogel, Insulin, Maltodextrin, Sodium alginate",

author = "Haoran Cheng and Haishan Wu and Ting Guo and {Jin Park}, Hyun and Jinglei Li",

note = "Funding Information: This work is supported by National Natural Science Foundation of China (31700015). Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

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doi = "10.1016/j.ejpb.2022.08.016",

language = "English",

volume = "179",

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journal = "European Journal of Pharmaceutics and Biopharmaceutics",

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TY - JOUR

T1 - Zinc insulin hexamer loaded alginate zinc hydrogel

T2 - Preparation, characterization and in vivo hypoglycemic ability

AU - Cheng, Haoran

AU - Wu, Haishan

AU - Guo, Ting

AU - Jin Park, Hyun

AU - Li, Jinglei

PY - 2022/10

Y1 - 2022/10

N2 - Alginate zinc hydrogel loaded with zinc insulin hexamer was prepared and characterized for oral insulin administration. The hydrogel was fabricated by dripping zinc insulin hexamer into sodium alginate solution and followed by crosslinking by zinc chloride. SEM image reveals the zinc insulin hexamer was integrated into the matrix of hydrogel. Zinc insulin hexamer loaded hydrogel shows no obvious cytotoxicity to both HT29 and Caco-2 cells. The developed hydrogel retards the burst release of insulin in simulated gastric fluid but promotes the release when in simulated intestinal fluid. In the diabetic mice, zinc insulin hexamer loaded alginate hydrogel demonstrates significant and prolonged hypoglycemic effect.

AB - Alginate zinc hydrogel loaded with zinc insulin hexamer was prepared and characterized for oral insulin administration. The hydrogel was fabricated by dripping zinc insulin hexamer into sodium alginate solution and followed by crosslinking by zinc chloride. SEM image reveals the zinc insulin hexamer was integrated into the matrix of hydrogel. Zinc insulin hexamer loaded hydrogel shows no obvious cytotoxicity to both HT29 and Caco-2 cells. The developed hydrogel retards the burst release of insulin in simulated gastric fluid but promotes the release when in simulated intestinal fluid. In the diabetic mice, zinc insulin hexamer loaded alginate hydrogel demonstrates significant and prolonged hypoglycemic effect.

KW - Control release

KW - Hydrogel

KW - Insulin

KW - Maltodextrin

KW - Sodium alginate

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M3 - Article

C2 - 36087882

AN - SCOPUS:85138022735

SN - 0939-6411

VL - 179

SP - 173

EP - 181

JO - European Journal of Pharmaceutics and Biopharmaceutics

JF - European Journal of Pharmaceutics and Biopharmaceutics

ER -

Zinc insulin hexamer loaded alginate zinc hydrogel: Preparation, characterization and in vivo hypoglycemic ability

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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