In this study, several surface functionalisation techniques were used to immobilise β-galactosidase in a microreactor. β-Galactosidase was pretreated with lactose before immobilisation, and functionalised multi-walled carbon nanotubes (MWNTs), DNA-wrapped single-walled carbon nanotubes and glutaraldehyde as a linker were used to immobilise β-galactosidase on a microchannel surface. When functionalised MWNTs were used as a linker for immobilisation of pretreated β-galactosidase, the enzyme microreactor showed the highest performance. The K m(app) and V max were 2.84 mM and 0.52 mM/min, respectively, and the conversion of o-nitrophenyl-β-d-galactopyranoside (ONPG) reached 78.3% during the continuous flow reaction at a flow rate of 2.5 μL/min. In an enzyme microreactor, continuous synthesis of lactulose was performed, and the lactulose concentration was maintained at about 1.29 g/L for 48 h.
Bibliographical noteFunding Information:
This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea government (MEST) (No. 20100027563 ) and the Advanced Biomass R&D Center (ABC-2010-0029799) of Korea Grant funded by the Ministry of Education, Science and Technology.
- Enzyme microreactor
- Functionalised multi-walled carbon nanotubes
ASJC Scopus subject areas
- Analytical Chemistry
- Food Science